A new risk variant for multiple sclerosis at 11q23.3 locus is associated with expansion of CXCR5+ circulating regulatory T cells

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  • dc.contributor.author Gil-Varea, Elia
  • dc.contributor.author Bosch Fusté, Elena
  • dc.contributor.author Navarro i Cuartiellas, Arcadi, 1969-
  • dc.contributor.author Comabella López, Manuel
  • dc.date.accessioned 2020-05-06T09:30:59Z
  • dc.date.available 2020-05-06T09:30:59Z
  • dc.date.issued 2020
  • dc.description.abstract Genome-wide association studies and meta-analysis have contributed to the identification of more than 200 loci associated with multiple sclerosis (MS). However, a proportion of MS heritability remains unknown. We aimed to uncover new genetic variants associated with MS and determine their functional effects. For this, we resequenced the exons and regulatory sequences of 14 MS risk genes in a cohort of MS patients and healthy individuals (n = 1,070) and attempted to validate a selection of signals through genotyping in an independent cohort (n = 5,138). We identified three new MS-associated variants at C-X-C motif chemokine receptor 5 (CXCR5), Ts translation elongation factor, mitochondrial (TSFM) and cytochrome P450 family 24 subfamily A member 1 (CYP24A1). Rs10892307 resulted in a new signal at the CXCR5 region that explains one of the associations with MS within the locus. This polymorphism and three others in high linkage disequilibrium mapped within regulatory regions. Of them, rs11602393 showed allele-dependent enhancer activity in the forward orientation as determined by luciferase reporter assays. Immunophenotyping using peripheral blood mononuclear cells from MS patients associated the minor allele of rs10892307 with increased percentage of regulatory T cells expressing CXCR5. This work reports a new signal for the CXCR5 MS risk locus and points to rs11602393 as the causal variant. The expansion of CXCR5+ circulating regulatory T cells induced by this variant could cause its MS association.
  • dc.description.sponsorship Funding: The genotyping service was carried out at CEGEN-PRB2-ISCIII and was supported by grant PT13/0001, ISCIII-SGEFI/FEDER. This work was also supported by the following grants: BFU2016-77961-P (AEI/FEDER); 2017-SGR-00702 (Direcció General de Recerca, Generalitat de Catalunya); Unidad de Excelencia María de Maeztu funded by the MINECO (MDM-2014-0370); Proyectos de Investigación en Salud (FIS PI12/02229, PI15/00587, PI16/01259); Red Española de Esclerosis Múltiple (RD16/0015/0004 to M.C., RD16/0015/0002; RD16/0015/0005 to K.V.; RD16/0015/0016) integrated in the Plan Estatal I+D+I and cofunded by Instituto de Salud Carlos III and the Fondo Europeo de Desarrollo Regional (FEDER; “Otra forma de hacer Europa”); SAF2016-80595-C2-1-P (Ministerio de Economía, Industria y Competitividad)
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Gil-Varea E, Fedetz M, Eixarch H, Spataro N, Villar LM, Urcelay E et al. A new risk variant for multiple sclerosis at 11q23.3 locus is associated with expansion of CXCR5+ circulating regulatory T cells. J Clin Med. 2020 Feb 26; 9(3). pii: E625. DOI: 10.3390/jcm9030625
  • dc.identifier.doi http://dx.doi.org/10.3390/jcm9030625
  • dc.identifier.issn 2077-0383
  • dc.identifier.uri http://hdl.handle.net/10230/44435
  • dc.language.iso eng
  • dc.publisher MDPI
  • dc.relation.ispartof Journal of Clinical Medicine. 2020 Feb 26; 9(3). pii: E625
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016-77961-P
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-80595-C2-1-P
  • dc.rights © 2020 by Elia Gil-Varea et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Esclerosi múltiple
  • dc.subject.other Genètica
  • dc.title A new risk variant for multiple sclerosis at 11q23.3 locus is associated with expansion of CXCR5+ circulating regulatory T cells
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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