Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network

Blume, Julia von; Alleaume, Anne-Marie; Kienzle, Christine; Carreras Sureda, Amado, 1986-; Valverde, M. A. (Miguel Ángel), 1963-; Malhotra, Vivek

doi:http://dx.doi.org/10.1083/jcb.201207180

Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network

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von Blume J, Alleaume AM, Kienzle C, Carreras-Sureda A, Valverde M, Malhotra V. Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network. J Cell Biol. 2012;199(7):1057-66. DOI: 10.1083/jcb.201207180

Abstract

Ca(2+) import into the lumen of the trans-Golgi network (TGN) by the secretory pathway calcium ATPase1 (SPCA1) is required for the sorting of secretory cargo. How is Ca(2+) retained in the lumen of the Golgi, and what is its role in cargo sorting? We show here that a soluble, lumenal Golgi resident protein, Cab45, is required for SPCA1-dependent Ca(2+) import into the TGN; it binds secretory cargo in a Ca(2+)-dependent reaction and is required for its sorting at the TGN.