The magic of unraveling genome architecture and function

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Cosma, Maria Pia
• dc.contributor.author Neguembor, Maria Victoria
• dc.date.accessioned 2023-06-16T06:11:15Z
• dc.date.available 2023-06-16T06:11:15Z
• dc.date.issued 2023
• dc.description.abstract Over the last decades, technological breakthroughs in super-resolution microscopy have allowed us to reach molecular resolution and design experiments of unprecedented complexity. Investigating how chromatin is folded in 3D, from the nucleosome level up to the entire genome, is becoming possible by "magic" (imaging genomic), i.e., the combination of imaging and genomic approaches. This offers endless opportunities to delve into the relationship between genome structure and function. Here, we review recently achieved objectives and the conceptual and technical challenges the field of genome architecture is currently undertaking. We discuss what we have learned so far and where we are heading. We elucidate how the different super-resolution microscopy approaches and, more specifically, live-cell imaging have contributed to the understanding of genome folding. Moreover, we discuss how future technical developments could address remaining open questions.
• dc.description.sponsorship We acknowledge the support from the European Union’s Horizon 2020 Research and Innovation Program (no. 964342 to M.P.C.); the Ministerio de Ciencia e Innovación (grant no. 008506-PID2020- 114080GB-I00 to M.P.C.); an AGAUR grant from Secretaria d’Universitats i Recerca del Departament d’Empresa iConeixement de la Generalitat de Catalunya (grant no. 006712 BFU2017-86760-P [AEI/FEDER, UE] to M.P.C.); the CERCA Program/Generalitat de Catalunya (to CRG); ICREA (Institucio Catalana de Recerca i Estudis Avançats) (to M.P.C.); the National Natural Science Foundation of China (31971177 and 32270577 to M.P.C.); Guangzhou Key Projects of Brain Science and Brain-Like Intelligence Technology (20200730009 to M.P.C.); Barcelona Institute of Science and Technology (BIST) Ignite Grants (Seeding Stage 2017 and Second Phase 2018, to M.V.N.); the People Program (Marie Curie Actions) FP7/2007–2013 under REA (grant no. 608959 to M.V.N.); and Juan de la Cierva-Incorporación 2017 (to M.V.N.) and the Spanish Ministry of Science and Innovation through the Centro de Excelencia Severo Ochoa (CEX2020-001049-S, MCIN/AEI/10.13039/501100011033 to CRG).
• dc.format.mimetype application/pdf
• dc.identifier.citation Cosma MP, Neguembor MV. The magic of unraveling genome architecture and function. Cell Rep. 2023 Apr 25;42(4):112361. DOI: 10.1016/j.celrep.2023.112361
• dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2023.112361
• dc.identifier.issn 2211-1247
• dc.identifier.uri http://hdl.handle.net/10230/57184
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Cell Rep. 2023 Apr 25;42(4):112361
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/964342
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-114080GB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-86760-P
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/608959
• dc.rights © 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword 3D genome folding
• dc.subject.keyword CP: Molecular biology
• dc.subject.keyword Chromatin conformation
• dc.subject.keyword Genome organization
• dc.subject.keyword Live imaging
• dc.subject.keyword Super-resolution imaging
• dc.title The magic of unraveling genome architecture and function
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion