Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brain

Albuixech Crespo, Beatriz; López Blanch, Laura; Burguera, Demián; Maeso, Ignacio; Sánchez Arrones, Luisa; Moreno Bravo, Juan Antonio; Somorjai, Ildikó Maureen Lara; Pascual Anaya, Juan; Puelles, Eduardo; Bovolenta, Paola; García Fernàndez, Jordi; Puelles, Luis; Irimia Martínez, Manuel; Ferrán, José Luis E.

Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brain

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dc.contributor.author Albuixech Crespo, Beatrizca
dc.contributor.author López Blanch, Lauraca
dc.contributor.author Burguera, Demiánca
dc.contributor.author Maeso, Ignacioca
dc.contributor.author Sánchez Arrones, Luisaca
dc.contributor.author Moreno Bravo, Juan Antonioca
dc.contributor.author Somorjai, Ildikó Maureen Laraca
dc.contributor.author Pascual Anaya, Juanca
dc.contributor.author Puelles, Eduardoca
dc.contributor.author Bovolenta, Paolaca
dc.contributor.author García Fernàndez, Jordica
dc.contributor.author Puelles, Luisca
dc.contributor.author Irimia Martínez, Manuelca
dc.contributor.author Ferrán, José Luis E.ca
dc.date.accessioned 2018-07-02T07:45:49Z
dc.date.available 2018-07-02T07:45:49Z
dc.date.issued 2017
dc.description.abstract All vertebrate brains develop following a common Bauplan defined by anteroposterior (AP) and dorsoventral (DV) subdivisions, characterized by largely conserved differential expression of gene markers. However, it is still unclear how this Bauplan originated during evolution. We studied the relative expression of 48 genes with key roles in vertebrate neural patterning in a representative amphioxus embryonic stage. Unlike nonchordates, amphioxus develops its central nervous system (CNS) from a neural plate that is homologous to that of vertebrates, allowing direct topological comparisons. The resulting genoarchitectonic model revealed that the amphioxus incipient neural tube is unexpectedly complex, consisting of several AP and DV molecular partitions. Strikingly, comparison with vertebrates indicates that the vertebrate thalamus, pretectum, and midbrain domains jointly correspond to a single amphioxus region, which we termed Di-Mesencephalic primordium (DiMes). This suggests that these domains have a common developmental and evolutionary origin, as supported by functional experiments manipulating secondary organizers in zebrafish and mice.
dc.description.sponsorship Spanish Ministry of Economy and Competitiveness and European FEDER funds (grant number BFU2014-57516-P). To Luis Puelles and Jose Luis Ferran. European Research Council (grant number ERC-StG-LS2-637591). To Manuel Irimia. Spanish Ministry of Economy and Competitiveness (grant number SEV-2012-0208).Centro de Excelencia Severo Ochoa (to CRG, Manuel Irimia). Spanish Ministry of Economy and Competitiveness (grant number BFU2014-58908-P). To Jordi Garcia-Fernadez. Seneca Foundation, Comunidad de Murcia (grant number 19904/GERM/15). To Luis Puelles. Generalitat de Catalunya (grant number). ICREA Academia Prize to Jordi Garcia-Fernandez. Spanish Ministry of Economy and Competitiveness (grant number BFU2013-43213-P). To Paola Bovolenta. Spanish Ministry of Economy and Competitiveness (grant number BFU2014-55076-P). To Manuel Irimia. Including an FPI PhD fellowship to Laura Lopez-Blanch. Marine Alliance for Science and Technology Scotland (MASTS) (grant number). To Ildiko Somorjai.
dc.format.mimetype application/pdf
dc.identifier.citation Albuixech-Crespo B, López-Blanch L, Burguera D, Maeso I, Sánchez-Arrones L, Moreno-Bravo JA et al. Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brain. PLoS Biol. 2017 Apr 19;15(4):e2001573. DOI: 10.1371/journal.pbio.2001573
dc.identifier.doi http://dx.doi.org/10.1371/journal.pbio.2001573
dc.identifier.issn 1544-9173
dc.identifier.uri http://hdl.handle.net/10230/35010
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)ca
dc.relation.ispartof PLOS Biology. 2017 Apr 19;15(4):e2001573
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/637591
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-57516-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-58908-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-43213-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-55076-P
dc.rights © 2017 Albuixech-Crespo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.keyword Amphioxus
dc.subject.keyword Gene expression
dc.subject.keyword In situ hybridization
dc.subject.keyword Chick embryo
dc.subject.keyword Body patterning
dc.subject.keyword Neural tube
dc.title Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brainca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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