Reduced airway levels of fatty-acid binding protein 4 in COPD: relationship with airway infection and disease severity

Perea, Lídia; Rodrigo-Troyano, Ana; Cantó, Elisabet; Domínguez Álvarez, Marisol; Giner, Jordi; Sanchez-Reus, Ferran; Villar García, Judit; Quero, Sara; García-Nuñez, Marian; Marín, Alicia; Monsó Molas, Eduard; Faner, Rosa; Vidal, Silvia; Sibila, Oriol

Reduced airway levels of fatty-acid binding protein 4 in COPD: relationship with airway infection and disease severity

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dc.contributor.author Perea, Lídia
dc.contributor.author Rodrigo-Troyano, Ana
dc.contributor.author Cantó, Elisabet
dc.contributor.author Domínguez Álvarez, Marisol
dc.contributor.author Giner, Jordi
dc.contributor.author Sanchez-Reus, Ferran
dc.contributor.author Villar García, Judit
dc.contributor.author Quero, Sara
dc.contributor.author García-Nuñez, Marian
dc.contributor.author Marín, Alicia
dc.contributor.author Monsó Molas, Eduard
dc.contributor.author Faner, Rosa
dc.contributor.author García-Nuñez, Marian
dc.contributor.author Vidal, Silvia
dc.contributor.author Sibila, Oriol
dc.date.accessioned 2020-06-19T07:06:41Z
dc.date.available 2020-06-19T07:06:41Z
dc.date.issued 2020
dc.description.abstract Background: For still unclear reasons, chronic airway infection often occurs in patients with Chronic Obstructive Pulmonary Disease (COPD), particularly in those with more severe airflow limitation. Fatty-acid binding protein 4 (FABP4) is an adipokine involved in the innate immune response against infection produced by alveolar macrophages (Mɸ). We hypothesized that airway levels of FABP4 may be altered in COPD patients with chronic airway infection. Methods: In this prospective and controlled study we: (1) compared airway FABP4 levels (ELISA) in induced sputum, bronchoalveolar lavage fluid (BALF) and plasma samples in 52 clinically stable COPD patients (65.2 ± 7.9 years, FEV1 59 ± 16% predicted) and 29 healthy volunteers (55.0 ± 12.3 years, FEV1 97 ± 16% predicted); (2) explored their relationship with the presence of bacterial airway infection, defined by the presence of potentially pathogenic bacteria (PPB) at ≥103 colony-forming units/ml in BALF; (3) investigated their relationship with the quantity and proportion of Mɸ in BALF (flow cytometry); and, (4) studied their relationship with the severity of airflow limitation (FEV1), GOLD grade and level of symptoms (CAT questionnaire). Results: We found that: (1) airway levels of FABP4 (but not plasma ones) were reduced in COPD patients vs. controls [219.2 (96.0-319.6) vs. 273.4 (203.1-426.7) (pg/ml)/protein, p = 0.03 in BALF]; (2) COPD patients with airway infection had lower sputum FABP4 levels [0.73 (0.35-15.3) vs. 15.6 (2.0-29.4) ng/ml, p = 0.02]; (3) in COPD patients, the number and proportion of Mɸ were positively related with FABP4 levels in BALF; (4) BALF and sputum FABP4 levels were positively related with FEV1, negatively with the CAT score, and lowest in GOLD grade D patients. Conclusions: Airway FABP4 levels are reduced in COPD patients, especially in those with airway infection and more severe disease. The relationship observed between Mɸ and airway FABP4 levels supports a role for FABP4 in the pathogenesis of airway infection and disease severity in COPD.
dc.format.mimetype application/pdf
dc.identifier.citation Perea L, Rodrigo-Troyano A, Cantó E, Domínguez-Álvarez M, Giner J, Sanchez-Reus F, et al. Reduced airway levels of fatty-acid binding protein 4 in COPD: relationship with airway infection and disease severity. Respir Res. 2020 Jan 13 ;21(1):21. DOI: 10.1186/s12931-020-1278-5
dc.identifier.doi http://dx.doi.org/10.1186/s12931-020-1278-5
dc.identifier.issn 1465-9921
dc.identifier.uri http://hdl.handle.net/10230/45018
dc.language.iso eng
dc.publisher BioMed Central
dc.rights Copyright © The Author(s). 2020. Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.keyword Bronchoalveolar lavage fluid
dc.subject.keyword Chronic obstructive pulmonary disease
dc.subject.keyword FABP4
dc.subject.keyword Macrophages
dc.title Reduced airway levels of fatty-acid binding protein 4 in COPD: relationship with airway infection and disease severity
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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