(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

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  • dc.contributor.author Neguembor, Maria Victoria
  • dc.contributor.author Sebastián Pérez, Rubén
  • dc.contributor.author Aulicino, Francesco, 1987-
  • dc.contributor.author Gómez García, Pablo
  • dc.contributor.author Cosma, Maria Pia, 1970-
  • dc.contributor.author Lakadamyali, Melike
  • dc.date.accessioned 2019-11-27T08:34:21Z
  • dc.date.available 2019-11-27T08:34:21Z
  • dc.date.issued 2018
  • dc.description.abstract CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization using super-resolution microscopy. We developed (Po)STAC (Polycistronic SunTAg modified CRISPR) by combining CRISPR/dCas9 with SunTag labeling and polycistronic vectors. (Po)STAC enhances both labeling efficiency and fluorescence signal detected from labeled loci enabling live cell imaging as well as super-resolution fixed-cell imaging of multiple genes with high spatiotemporal resolution.
  • dc.description.sponsorship European Union’s Horizon 2020 Research and Innovation Programme [CellViewer No 686637 to M.L., M.P.C.]; Ministerio de Economia y Competitividad [BFU2013–49867-EXP to M.L., M.P.C.]; Fundació Cellex Barcelona (to M.L); European Union Seventh Framework Programme under the European Research Council Grants [337191-MOTORS to M.L.]; ‘Severo Ochoa’ Programme for Centres of Excellence in R&D [SEV-2015- 0522 to M.L.]; Ministerio de Economia y Competitividad and FEDER Funds [BFU2014–54717-P, BFU2015–71984-ERC to M.P.C.]; AGAUR Grant [2014 SGR1137 to M.P.C.]; Spanish Ministry of Economy and Competitiveness (to M.P.C.); Centro de Excelencia Severo Ochoa [2013–2017 to M.P.C.]; CERCA Programme/Generalitat de Catalunya (to M.P.C); Ministerio de Ciencia e Innovacion FPI (to F.A.); People Program (Marie Curie Actions) FP7/2007–2013 under REA grant [608959 to M.V.N.]. Funding for open access charge: European Union’s Horizon 2020 Research and Innovation Programme [CellViewer No 686637].
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Nucleic Acids Res. 2018; 46(5):e30. DOI 10.1093/nar/gkx1271
  • dc.identifier.doi http://dx.doi.org/10.1093/nar/gkx1271
  • dc.identifier.issn 0305-1048
  • dc.identifier.uri http://hdl.handle.net/10230/43001
  • dc.language.iso eng
  • dc.publisher Oxford University Press
  • dc.relation.ispartof Nucleic Acids Research. 2018;46(5):e30
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/686637
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013–49867-EXP
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014–54717-P
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015–71984-ERC
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/608959
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/686637
  • dc.rights © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
  • dc.subject.keyword Cell biology
  • dc.subject.keyword Targeted gene modification
  • dc.title (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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