(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

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dc.contributor.authorNeguembor, Maria Victoria
dc.contributor.authorSebastián Pérez, Rubén
dc.contributor.authorAulicino, Francesco, 1987-
dc.contributor.authorGómez García, Pablo
dc.contributor.authorCosma, Maria Pia, 1970-
dc.contributor.authorLakadamyali, Melike
dc.date.accessioned2019-11-27T08:34:21Z
dc.date.available2019-11-27T08:34:21Z
dc.date.issued2018
dc.description.abstractCRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization using super-resolution microscopy. We developed (Po)STAC (Polycistronic SunTAg modified CRISPR) by combining CRISPR/dCas9 with SunTag labeling and polycistronic vectors. (Po)STAC enhances both labeling efficiency and fluorescence signal detected from labeled loci enabling live cell imaging as well as super-resolution fixed-cell imaging of multiple genes with high spatiotemporal resolution.
dc.description.sponsorshipEuropean Union’s Horizon 2020 Research and Innovation Programme [CellViewer No 686637 to M.L., M.P.C.]; Ministerio de Economia y Competitividad [BFU2013–49867-EXP to M.L., M.P.C.]; Fundació Cellex Barcelona (to M.L); European Union Seventh Framework Programme under the European Research Council Grants [337191-MOTORS to M.L.]; ‘Severo Ochoa’ Programme for Centres of Excellence in R&D [SEV-2015- 0522 to M.L.]; Ministerio de Economia y Competitividad and FEDER Funds [BFU2014–54717-P, BFU2015–71984-ERC to M.P.C.]; AGAUR Grant [2014 SGR1137 to M.P.C.]; Spanish Ministry of Economy and Competitiveness (to M.P.C.); Centro de Excelencia Severo Ochoa [2013–2017 to M.P.C.]; CERCA Programme/Generalitat de Catalunya (to M.P.C); Ministerio de Ciencia e Innovacion FPI (to F.A.); People Program (Marie Curie Actions) FP7/2007–2013 under REA grant [608959 to M.V.N.]. Funding for open access charge: European Union’s Horizon 2020 Research and Innovation Programme [CellViewer No 686637].
dc.format.mimetypeapplication/pdf
dc.identifier.citationNeguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Nucleic Acids Res. 2018; 46(5):e30. DOI 10.1093/nar/gkx1271
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gkx1271
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/10230/43001
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofNucleic Acids Research. 2018;46(5):e30
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/686637
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BFU2013–49867-EXP
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BFU2014–54717-P
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BFU2015–71984-ERC
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/608959
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/686637
dc.rights© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subject.keywordCell biology
dc.subject.keywordTargeted gene modification
dc.title(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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