Wnt controls the transcriptional activity of Kaiso through CK1ε-dependent phosphorylation of p120-catenin

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  • dc.contributor.author Valle Pérez, Beatriz delca
  • dc.contributor.author Casagolda, Davidca
  • dc.contributor.author Lugilde, Eroca
  • dc.contributor.author Valls, Gabrielaca
  • dc.contributor.author Codina, Montserratca
  • dc.contributor.author Dave, Natàliaca
  • dc.contributor.author García de Herreros, Antonioca
  • dc.contributor.author Duñach, Mireiaca
  • dc.date.accessioned 2015-12-18T18:55:36Z
  • dc.date.available 2015-12-18T18:55:36Z
  • dc.date.issued 2011
  • dc.description.abstract p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. In response to Wnt3a, p120-catenin is phosphorylated at Ser268 and Ser269, disrupting its interaction with E-cadherin. Here, we describe that Wnt-induced p120-catenin phosphorylation at Ser268 and Ser269 also enhances its binding to the transcriptional factor Kaiso, preventing Kaiso-mediated inhibition of the β-catenin-Tcf-4 transcriptional complex. Kaiso-mediated repression of this complex is due to its association not only with Tcf-4 but also with β-catenin. Disruption of Tcf-4-Kaiso and β-catenin-Kaiso interactions by p120-catenin not only releases Tcf-4 and β-catenin enabling its mutual association and the formation of the transcriptional complex but also permits Kaiso binding to methylated CpG islands, an interaction that is weakly inhibited by p120-catenin. Consequently, Wnt stimulates Kaiso association to the CDKN2A promoter, which contains CpG sequences, in cells where these sequences are extensively methylated, such as HT-29 M6, an effect accompanied by decreased expression of its gene product. These results indicate that, when released from E-cadherin by Wnt3a-stimulated phosphorylation, p120-catenin controls the activity of the Kaiso transcriptional factor, enhancing its binding to repressed promoters and relieving its inhibition of the β-catenin-Tcf-4 transcriptional complex.ca
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Del Valle-Pérez B, Casagolda D, Lugilde E, Valls G, Codina M, Dave N et al. Wnt controls the transcriptional activity of Kaiso through CK1ε-dependent phosphorylation of p120-catenin. Journal of cell science. 2011;124(Pt 13):2298-309. DOI: 10.1242/jcs.082693ca
  • dc.identifier.doi http://dx.doi.org/10.1242/jcs.082693
  • dc.identifier.issn 0021-9533
  • dc.identifier.uri http://hdl.handle.net/10230/25478
  • dc.language.iso engca
  • dc.publisher Company of Biologistsca
  • dc.relation.ispartof Journal of cell science. 2011;124(Pt 13):2298-309
  • dc.rights © Company of Biologists http://jcs.biologists.org/content/124/13/2298.long DOI 10.1242/jcs.082693ca
  • dc.rights.accessRights info:eu-repo/semantics/openAccessca
  • dc.subject.keyword Wnt signaling
  • dc.subject.keyword p120-catenin
  • dc.subject.keyword Kaiso
  • dc.subject.keyword CK1 phosphorylation
  • dc.subject.other Cadherinesca
  • dc.subject.other Proteïnes Fixacióca
  • dc.subject.other Fosforilacióca
  • dc.subject.other Factors de transcripcióca
  • dc.title Wnt controls the transcriptional activity of Kaiso through CK1ε-dependent phosphorylation of p120-cateninca
  • dc.type info:eu-repo/semantics/articleca
  • dc.type.version info:eu-repo/semantics/publishedVersionca