Identification and quantification of psychoactive drugs in whole blood using dried blood spot (DBS) by ultra-performance liquid chromatography tandem mass spectrometry.

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• dc.contributor.author Kyriakou, Chrystallaca
• dc.contributor.author Marchei, Emiliaca
• dc.contributor.author Scaravelli, Giuliaca
• dc.contributor.author García Algar, Oscarca
• dc.contributor.author Supervía Caparrós, Augustoca
• dc.contributor.author Graziano, Silviaca
• dc.date.accessioned 2016-09-29T09:05:06Z
• dc.date.issued 2016
• dc.description.abstract A procedure based on ultra-high-pressure liquid chromatography tandem mass spectrometry has been developed for the determination of twenty three psychoactive drugs and metabolites in whole blood using dried blood spot (DBS). Chromatographic separation was achieved at ambient temperature using a reverse-phase column and a linear gradient elution with two solvents: 0.1% formic acid in acetonitrile and 5mM ammonium formate at pH 3. The mass spectrometer was operated in positive ion mode, using multiple reaction monitoring via positive electro-spray ionization. The method was linear from the limit of quantification (5ng/ml for all the analytes apart from 15ng/ml for Δ-9-tetrahydrocannabinol and metabolites) to 500ng/ml, and showed good correlation coefficients (r(2)=0.990) for all substances. Analytical recovery of analytes under investigation was always higher than 75% and intra-assay and inter-assay precision and accuracy always better than 15%. Using the validated method, ten DBS samples, collected at the hospital emergency department in cases of acute drug intoxication, were found positive to one or more psychoactive drugs. Our data support the potential of DBS sampling for non invasive monitoring of exposure/intoxication to psychoactive drugs.ca
• dc.format.mimetype application/pdfca
• dc.identifier.citation Kyriakou C, Marchei E, Scaravelli G, García-Algar O, Supervía A, Graziano S. Identification and quantification of psychoactive drugs in whole blood using dried blood spot (DBS) by ultra-performance liquid chromatography tandem mass spectrometry. J Pharm Biomed Anal. 2016 Sep 5;128:53-60. doi: 10.1016/j.jpba.2016.05.011ca
• dc.identifier.doi http://dx.doi.org/10.1016/j.jpba.2016.05.011
• dc.identifier.issn 0731-7085
• dc.identifier.uri http://hdl.handle.net/10230/27320
• dc.language.iso engca
• dc.publisher Elsevierca
• dc.relation.ispartof Journal of Pharmaceutical and Biomedical Analysis. 2016 Sep 5;128:53-60
• dc.rights © Elsevier http://dx.doi.org/10.1016/j.jpba.2016.05.011ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.other Droguesca
• dc.subject.other Espectrometria de massesca
• dc.title Identification and quantification of psychoactive drugs in whole blood using dried blood spot (DBS) by ultra-performance liquid chromatography tandem mass spectrometry.ca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca