In vitro characterization of in situ alloyed Ti6Al4V(ELI)-3 at.% Cu obtained by laser powder bed fusion

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• dc.contributor.author Martín Vilardell, Anna
• dc.contributor.author Krakhmalev, Pavel
• dc.contributor.author Yadroitsava, Ina
• dc.contributor.author Yadroitsev, Igor
• dc.contributor.author García-Giralt, Natalia
• dc.date.accessioned 2022-09-13T06:42:35Z
• dc.date.available 2022-09-13T06:42:35Z
• dc.date.issued 2021
• dc.description.abstract The intensive cytotoxicity of pure copper is effectively kills bacteria, but it can compromise cellular behavior, so a rational balance must be found for Cu-loaded implants. In the present study, the individual and combined effect of surface composition and roughness on osteoblast cell behavior of in situ alloyed Ti6Al4V(ELI)-3 at.% Cu obtained by laser powder bed fusion was studied. Surface composition was studied using scanning electron microscopy, energy dispersive spectroscopy, and X-ray diffraction. Surface roughness measurements were carried out using confocal microscopy. In vitro osteoblast performance was evaluated by means of cell morphology observation of cell viability, proliferation, and mineralization. In vitro studies were performed at 1, 7, and 14 days of cell culture, except for cell mineralization at 28 days, on grounded and as-built (rough) samples with and without 3 at.% Cu. The addition of 3 at.% Cu did not show cell cytotoxicity but inhibited cell proliferation. Cell mineralization tends to be higher for samples with 3 at.% Cu content. Surface roughness inhibited cell proliferation too, but showed enhanced cell mineralization capacity and therefore, higher osteoblast performance, especially when as-built samples contained 3 at.% Cu. Cell proliferation was only observed on ground samples without Cu but showed the lowest cell mineralization.
• dc.format.mimetype application/pdf
• dc.identifier.citation Vilardell AM, Krakhmalev P, Yadroitsava I, Yadroitsev I, Garcia-Giralt N. et al. In vitro characterization of in situ alloyed Ti6Al4V(ELI)-3 at.% Cu obtained by laser powder bed fusion. Materials (Basel). 2021 Nov 27; 14(23):7260. DOI: 10.3390/ma14237260
• dc.identifier.doi http://dx.doi.org/10.3390/ma14237260
• dc.identifier.issn 1996-1944
• dc.identifier.uri http://hdl.handle.net/10230/54053
• dc.language.iso eng
• dc.publisher MDPI
• dc.rights © [AAAA] by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Ti–Cu alloys
• dc.subject.keyword Implants
• dc.subject.keyword In-vitro tests
• dc.subject.keyword Laser powder bed fusion
• dc.subject.keyword Surface roughness
• dc.title In vitro characterization of in situ alloyed Ti6Al4V(ELI)-3 at.% Cu obtained by laser powder bed fusion
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion