The immunobiogram, a novel in vitro assay to evaluate treatment resistance in patients receiving immunosuppressive therapy

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• dc.contributor.author Portolés, José
• dc.contributor.author Jimenez, Carlos
• dc.contributor.author Janeiro, Dario
• dc.contributor.author López-Oliva, Maria O.
• dc.contributor.author Ortega-Carrión, Álvaro
• dc.contributor.author Blanquez, Daniel
• dc.contributor.author Arribas, Luis
• dc.contributor.author Gómez, Carlos
• dc.contributor.author Díez, Teresa
• dc.contributor.author Pascual Santos, Julio
• dc.contributor.author Portero, Isabel
• dc.date.accessioned 2022-06-08T07:48:02Z
• dc.date.available 2022-06-08T07:48:02Z
• dc.date.issued 2021
• dc.description.abstract Immunosuppressive drugs are widely used to treat several autoimmune disorders and prevent rejection after organ transplantation. However, intra-individual variations in the pharmacological response to immunosuppressive therapy critically influence its efficacy, often resulting in poor treatment responses and serious side effects. Effective diagnostic tools that help clinicians to tailor immunosuppressive therapy to the needs and immunological profile of the individual patient thus constitute a major unmet clinical need. In vitro assays that measure immune cell responses to immunosuppressive drugs constitute a promising approach to individualized immunosuppressive therapy. Here, we present the Immunobiogram, a functional pharmacodynamic immune cell-based assay for simultaneous quantitative measurement of a patient's immune response to a battery of immunosuppressive drugs. Peripheral blood mononuclear cells collected from patients are immunologically stimulated to induce activation and proliferation and embedded in a hydrogel mixture in which they are exposed to a concentration gradient of the immunosuppressants of interest. Analysis of samples from kidney transplant patients using this procedure revealed an association between the sensitivity of individual patients to the immunosuppressive regimen and their immunological risk of transplant rejection. Incorporation of the Immunobiogram assay into clinical settings could greatly facilitate personalized optimization and monitoring of immunosuppressive therapy, and study of the mechanisms underlying resistance to immunosuppressants.
• dc.description.sponsorship Research was funded by a competitive grant from the European Commission (SME Instrument – Phase II), Project: TRANSBIO (Id 733248) “Cellular BIOtechnology for prognosis and monitoring in renal TRANSplantation” (https://cordis.europa.eu/project/id/733248).
• dc.format.mimetype application/pdf
• dc.identifier.citation Portoles JM, Jimenez C, Janeiro D, Lopez-Oliva MO, Ortega-Carrion A, Blanquez D, et al. The immunobiogram, a novel in vitro assay to evaluate treatment resistance in patients receiving immunosuppressive therapy. Front Immunol. 2021 Jan 25; 11: 618202. DOI: 10.3389/fimmu.2020.618202
• dc.identifier.doi http://dx.doi.org/10.3389/fimmu.2020.618202
• dc.identifier.issn 1664-3224
• dc.identifier.uri http://hdl.handle.net/10230/53406
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/733248
• dc.rights Copyright © 2021 Portoles, Jimenez, Janeiro, Lopez-Oliva, Ortega-Carrion, Blanquez, Arribas, Gomez, Diez, Pascual and Portero. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).https://creativecommons.org/licenses/by/4.0/. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cellular pharmacodynamics
• dc.subject.keyword Immune cell assay
• dc.subject.keyword Immunosuppressive therapy monitoring
• dc.subject.keyword Personalized medicine
• dc.subject.keyword Transplant rejection
• dc.title The immunobiogram, a novel in vitro assay to evaluate treatment resistance in patients receiving immunosuppressive therapy
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion