Innate cell-mediated cytotoxic activity of European sea bass leucocytes against nodavirus-infected cells: a functional and RNA-seq study

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  • dc.contributor.author Chaves Pozo, Elenaca
  • dc.contributor.author Valero, Yulemaca
  • dc.contributor.author Esteve-Codina, Annaca
  • dc.contributor.author Gómez Garrido, Jèssicaca
  • dc.contributor.author Dabad, Marcca
  • dc.contributor.author Alioto, Tylerca
  • dc.contributor.author Meseguer, Joséca
  • dc.contributor.author Esteban, María Ángelesca
  • dc.contributor.author Cuesta, Albertoca
  • dc.date.accessioned 2018-07-17T07:37:59Z
  • dc.date.available 2018-07-17T07:37:59Z
  • dc.date.issued 2017
  • dc.description.abstract Nervous necrosis virus (NNV) causes high mortalities in several marine species. We aimed to evaluate the innate cell-mediated cytotoxic (CMC) activity of head-kidney leucocytes (HKLs) isolated from naïve European sea bass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata), a very susceptible and resistant fish species to NNV, respectively, against fish cell lines infected with NNV. Seabream HKLs showed significantly increased innate CMC activity against NNV-infected cells, compared to those uninfected, while sea bass HKLs failed to do so. Thus, we performed a RNA-seq study to identify genes related to the CMC activity of sea bass leucocytes. Thus, we found that sea bass HKLs incubated with DLB-1 cells alone (CMC_DLB1) or with NNV-infected DLB-1 cells (CMC_DLB1-NNV) showed very similar transcriptomic profiles and the GO analysis revealed that most of the up-regulated genes were related to immunity. Strikingly, when the CMC samples with and without NNV were compared, GO analysis revealed that most of the up-regulated genes in CMC_DLB1-NNV samples were related to metabolism and very few to immunity. This is also in agreement with the functional data. These data point to the escape of CMC activity by NNV infection as an important factor involved in the high susceptibility to nodavirus infections of European sea bass.
  • dc.description.sponsorship This work was supported by grants of the National Bioinformatics Institute (INB), PRB2-ISCIII (PT13/0001/0044 to JG and AE); MINECO (PTA2014‐09515 to MD), MINECO and FEDER (AGL2013-43588-P and AGL2016-74866-C3-1-R to AC) and Fundación Séneca (Grupo de Excelencia de la Región de Murcia 19883/GERM/15). Nodavirus strain and SSN-1 cells were graciously provided by Pilar Fernández Somalo (Laboratorio Central de Veterinaria de Algete, Ministerio de Medio Ambiente, Rural y Marino).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Chaves-Pozo E, Valero Y, Esteve-Codina A, Gómez-Garrido J, Dabad M, Alioto T et al. Innate Cell-Mediated Cytotoxic Activity of European Sea Bass Leucocytes Against Nodavirus-Infected Cells: A Functional and RNA-seq Study. Sci Rep. 2017 Nov 13;7(1):15396. DOI: 10.1038/s41598-017-15629-6
  • dc.identifier.doi http://dx.doi.org/10.1038/s41598-017-15629-6
  • dc.identifier.issn 2045-2322
  • dc.identifier.uri http://hdl.handle.net/10230/35171
  • dc.language.iso eng
  • dc.publisher Nature Publishing Groupca
  • dc.relation.ispartof Scientific Reports. 2017 Nov 13;7(1):15396
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/PTA2014‐09515
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2013-43588-P
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2016-74866-C3-1-R
  • dc.rights © The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Innate immune cells
  • dc.subject.keyword Transcriptomics
  • dc.subject.keyword Virology
  • dc.title Innate cell-mediated cytotoxic activity of European sea bass leucocytes against nodavirus-infected cells: a functional and RNA-seq studyca
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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