Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation

Aranda Aragón, Sergio; Alcaine Colet, Anna, 1992-; Blanco, Enrique; Borràs, Eva; Caillot, Claire; Sabidó Aguadé, Eduard, 1981-; Di Croce, Luciano

doi:http://dx.doi.org/10.1126/sciadv.aav2448

Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation

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Aranda S, Alcaine-Colet A, Blanco E, Borràs E, Caillot C, Sabidó E et al. Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation. Sci Adv. 2019 Mar 6;5(3):eaav2448. DOI: 10.1126/sciadv.aav2448

Abstract

Profiling the chromatin-bound proteome (chromatome) in a simple, direct, and reliable manner might be key to uncovering the role of yet uncharacterized chromatin factors in physiology and disease. Here, we have designed an experimental strategy to survey the chromatome of proliferating cells by using the DNA-mediated chromatin pull-down (Dm-ChP) technology. Our approach provides a global view of cellular chromatome under normal physiological conditions and enables the identification of chromatin-bound proteins de novo. Integrating Dm-ChP with genomic and functional data, we have discovered an unexpected chromatin function for adenosylhomocysteinase, a major one-carbon pathway metabolic enzyme, in gene activation. Our study reveals a new regulatory axis between the metabolic state of pluripotent cells, ribosomal protein production, and cell division during the early phase of embryo development, in which the metabolic flux of methylation reactions is favored in a local milieu.