TOR and MAP kinase pathways synergistically regulate autophagy in response to nutrient depletion in fission yeast

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• dc.contributor.author Corral Ramos, Cristina
• dc.contributor.author Barrios, Rubén
• dc.contributor.author Ayté del Olmo, José
• dc.contributor.author Hidalgo Hernando, Elena
• dc.date.accessioned 2021-06-28T07:39:24Z
• dc.date.issued 2022
• dc.description.abstract General autophagy is an evolutionarily conserved process in eukaryotes, by which intracellular materials are transported into and degraded inside lysosomes or vacuoles, with the main goal of recycling those materials during periods of starvation. The molecular bases of autophagy have been widely described in Saccharomyces cerevisiae, and the specific roles of Atg proteins in the process were first characterized in this model system. Important contributions have been made in Schizosaccharomyces pombe highlighting the evolutionary similarity and, at the same time, diversity of Atg components in autophagy. However, little is known regarding signals, pathways and role of autophagy in this distant yeast. Here, we undertake a global approach to investigate the signals, the pathways and the consequences of autophagy activation. We demonstrate that not only nitrogen but several nutritional deprivations including lack of carbon, sulfur, phosphorus or leucine sources, trigger autophagy, and that the TORC1, TORC2 and MAP kinase Sty1 pathways control the onset of autophagy. Furthermore, we identify an unexpected phenotype of autophagy-defective mutants, namely their inability to survive in the absence of leucine when biosynthesis of this amino acid is impaired.Abbreviations: ATG: autophagy-related; cAMP: cyclic adenosine monophosphate; cDNA: complementary deoxyribonucleic acid; GFP: green fluorescence protein; Gluc: glucose; Leu: leucine; MAP: mitogen-activated protein; MM: minimal medium; PI: propidium iodine; PKA: protein kinase A; RNA: ribonucleic acid; RT-qPCR: real time quantitative polymerase chain reaction; S. cerevisiae: Saccharomyces cerevisiae; S. pombe: Schizosaccharomyces pombe; TCA: trichloroacetic acid; TOR: target of rapamycin; TORC1: target of rapamycin complex 1; TORC2: target of rapamycin complex 2; YE5S: yeast extract 5 amino acid supplemented.
• dc.description.sponsorship We thank Kaoru Takegawa for kindly providing plasmid pGFP-Atg8 in pREP41 and strain SK1, and Akio Nakashima and Fuyuhiko Tamanoi for providing AN0175, AN0179 and JUP1350 strains. We thank Li-Lin Du for helpful discussions. This work was supported by the Ministerio de Ciencia, Innovación y Universidades, PLAN E and FEDER (Spain) (PGC2018-093920-B-I00 to E.H.). The Oxidative Stress and Cell Cycle group is also supported by Generalitat de Catalunya (Spain) (2017-SGR-539) and by Unidad de Excelencia María de Maeztu, funded by the AEI (Spain) (CEX2018-000792-M). R.B. is recipient of a FPI contract from the Ministerio de Ciencia, Innovación y Universidades (Spain). E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain).
• dc.format.mimetype application/pdf
• dc.identifier.citation Corral-Ramos C, Barrios R, Ayté J, Hidalgo E. TOR and MAP kinase pathways synergistically regulate autophagy in response to nutrient depletion in fission yeast. Autophagy. 2022 Feb;18(2):375-90. DOI: 10.1080/15548627.2021.1935522
• dc.identifier.doi http://dx.doi.org/10.1080/15548627.2021.1935522
• dc.identifier.issn 1554-8627
• dc.identifier.uri http://hdl.handle.net/10230/47986
• dc.language.iso eng
• dc.publisher Taylor & Francis
• dc.relation.ispartof Autophagy. 2022 Feb;18(2):375-390
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00
• dc.rights © This is an Accepted Manuscript of an article published by Taylor & Francis in Autophagy on 2021 Jun 23, available online: http://www.tandfonline.com/10.1080/15548627.2021.1935522.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Autophagy
• dc.subject.keyword Gfp-Atg8
• dc.subject.keyword Sty1
• dc.subject.keyword Torc1
• dc.subject.keyword Torc2
• dc.subject.keyword Map kinase
• dc.subject.keyword Nutrient starvation
• dc.title TOR and MAP kinase pathways synergistically regulate autophagy in response to nutrient depletion in fission yeast
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion