Specific IgA and metalloproteinase activity in bronchial secretions from stable chronic obstructive pulmonary disease patients colonized by Haemophilus influenzae

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• dc.contributor.author Millares, Lauraca
• dc.contributor.author Marin, Aliciaca
• dc.contributor.author García Aymerich, Judithca
• dc.contributor.author Sauleda Roig, Jaumeca
• dc.contributor.author Belda, Joséca
• dc.contributor.author Monsó Molas, Eduardca
• dc.contributor.author PAC-COPD Study Groupca
• dc.date.accessioned 2015-03-16T08:16:08Z
• dc.date.available 2015-03-16T08:16:08Z
• dc.date.issued 2012ca
• dc.description.abstract Background: Haemophilus influenzae is the most common colonizing bacteria of the bronchial tree in chronic obstructive pulmonary disease (COPD), and positive cultures for this potentially pathogenic microorganism (PPM) has been associated with local inflammation changes that may influence the relationships between H. influenzae and the bronchial mucosa. Methods: A cross-sectional analysis of stable COPD patients enrolled in the Phenotype and Course of Chronic Obstructive Pulmonary Disease (PAC-COPD) Study, focusing on bronchial colonization by H. influenzae, was performed. Specific IgA against the PPM was measured by optical density, and metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) using ELISA in sputum samples. Levels in patients colonized by H. influenzae and non-colonized patients were compared. Results: Sputum supernatant for the measurement of specific IgA against H. influenzae was available from 54 stable COPD patients, who showed levels of specific IgA significantly lower in colonized (n=21) than in non-colonized patients (n=33) (15 [4-37] versus 31 [10-75], p=0.033, Mann-Whitney U test). Proenzyme MMP-9 was measured in 44 patients, and it was higher in colonized (n=12, 1903 [1488-6699] ng/ml) than in non-colonized patients (n=32, 639 [373-972] ng/ml) (p<0.001, Mann-Whitney U test). Active form of MMP-9 was also higher in colonized (126 [25-277] ng/ml) than in non-colonized patients (39 [14-68] ng/ml) (p=0.021, Mann-Whitney U test), and the molar ratio between proenzyme MMP-9 and TIMP-1 was above 1 (2.1 [0.1-12.5]) in colonized patients, significantly higher than the ratio found in non-colonized patients (0.2 [0.08-0.5]) (p=0.030, Mann-Whitney U test). Conclusions: Clinically stable COPD patients colonized by H. influenzae had lower levels of specific IgA against the microorganism and higher values of the active form of MMP-9 in their sputum supernatant than non-colonized patients. Bronchial colonization by H. influenzae may cause structural changes in the extracellular matrix through a defective defense and the production of active metalloproteinases.en
• dc.description.sponsorship This study was funded by Fondo de Investigación Sanitaria (PI060684/PI0901612), Fundació Parc Taulí, Societat Catalana de Pneumologia and CIBER de Enfermedades Respiratorias – CIBERES. CIBER de Enfermedades Respiratorias, CIBERES is an initiative of Instituto de Salud Carlos IIIen
• dc.format.mimetype application/pdfca
• dc.identifier.citation Millares L, Marin A, Garcia-Aymerich J, Sauleda J, Belda J, Monsó E; PAC-COPD Study Group. Specific IgA and metalloproteinase activity in bronchial secretions from stable chronic obstructive pulmonary disease patients colonized by Haemophilus influenzae. Respiratory Research. 2012; 13: 113. DOI 10.1186/1465-9921-13-113ca
• dc.identifier.doi http://dx.doi.org/10.1186/1465-9921-13-113
• dc.identifier.issn 1465-9921ca
• dc.identifier.uri http://hdl.handle.net/10230/23182
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof Respiratory Research. 2012; 13: 113
• dc.rights © 2012 Millares et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.keyword Chronic Obstructive Pulmonary Disease (COPD)en
• dc.subject.keyword Haemophilus influenzaeen
• dc.subject.keyword Secretory IgAen
• dc.subject.keyword Metalloproteinase-9 (MMP-9)en
• dc.subject.keyword Tissue-inhibitor of metalloproteinases-1 (TIMP-1)en
• dc.subject.other Pulmons -- Malalties obstructivesca
• dc.subject.other Immunoglobulinesca
• dc.title Specific IgA and metalloproteinase activity in bronchial secretions from stable chronic obstructive pulmonary disease patients colonized by Haemophilus influenzaeca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca