Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids

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  • dc.contributor.author Bustamante Madrid, Pilar
  • dc.contributor.author Barbáchano, Antonio
  • dc.contributor.author Albandea Rodríguez, David
  • dc.contributor.author Rodríguez Cobos, Javier
  • dc.contributor.author Rodríguez Salas, Nuria
  • dc.contributor.author Prieto Nieto, Isabel
  • dc.contributor.author Burgos García, Aurora
  • dc.contributor.author Martínez de Villarreal, Jaime
  • dc.contributor.author Real, Francisco X.
  • dc.contributor.author González Sancho, José Manuel
  • dc.contributor.author Larriba, María Jesús
  • dc.contributor.author Lafarga, Miguel
  • dc.contributor.author Muñoz, Alberto
  • dc.contributor.author Fernández Barral, Asunción
  • dc.date.accessioned 2024-06-11T06:13:28Z
  • dc.date.available 2024-06-11T06:13:28Z
  • dc.date.issued 2024
  • dc.description.abstract Understanding the mechanisms involved in colonic epithelial differentiation is key to unraveling the alterations causing inflammatory conditions and cancer. Organoid cultures provide an unique tool to address these questions but studies are scarce. We report a differentiation system toward enterocytes and goblet cells, the two major colonic epithelial cell lineages, using colon organoids generated from healthy tissue of colorectal cancer patients. Culture of these organoids in medium lacking stemness agents resulted in a modest ultrastructural differentiation phenotype with low-level expression of enterocyte (KLF4, KRT20, CA1, FABP2) and goblet cell (TFF2, TFF3, AGR2) lineage markers. BMP pathway activation through depletion of Noggin and addition of BMP4 resulted in enterocyte-biased differentiation. Contrarily, blockade of the Notch pathway using the γ-secretase inhibitor dibenzazepine (DBZ) favored goblet cell differentiation. Combination treatment with BMP4 and DBZ caused a balanced strong induction of both lineages. In contrast, colon tumor organoids responded poorly to BMP4 showing only weak signals of cell differentiation, and were unresponsive to DBZ. We also investigated the effects of 1α,25-dihydroxyvitamin D3 (calcitriol) on differentiation. Calcitriol attenuated the effects of BMP4 and DBZ on colon normal organoids, with reduced expression of differentiation genes and phenotype. Consistently, in normal organoids, calcitriol inhibited early signaling by BMP4 as assessed by reduction of the level of phospho-SMAD1/5/8. Our results show that BMP and Notch signaling play key roles in human colon stem cell differentiation to the enterocytic and goblet cell lineages and that calcitriol modulates these processes favoring stemness features.
  • dc.description.sponsorship This work was supported by grant PID2019-104867RB-I00 funded by MCIN/AEI/10.13039/501100011033; grant PID2022-136729OB-I00 funded by MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe”; grant S2022/BMD-7212 funded by Comunidad de Madrid; and grants ICI20/00057, CIBERONC/CB16/12/00273, CIBERONC/CB16/12/00453 and CIBERONC/CB16/12/00398 funded by the Instituto de Salud Carlos III - Fondo Europeo de Desarrollo Regional. PB-M and DA-R were supported by grants BES-2017-082483 and PRE2020-092713, respectively, funded by MCIN/AEI/10.13039/501100011033 and “ESF Investing in your future”. PB-M, AB, DA-R, JR-C, JMG-S, MJL, AM and AF-B belong to the Spanish National Research Council (CSIC)‘s Cancer Hub.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Bustamante-Madrid P, Barbáchano A, Albandea-Rodríguez D, Rodríguez-Cobos J, Rodríguez-Salas N, Prieto I, et al. Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids. Cell Death Dis. 2024 Apr 29;15(4):301. DOI: 10.1038/s41419-024-06680-z
  • dc.identifier.doi http://dx.doi.org/10.1038/s41419-024-06680-z
  • dc.identifier.issn 2041-4889
  • dc.identifier.uri http://hdl.handle.net/10230/60421
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Cell Death Dis. 2024 Apr 29;15(4):301
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-104867RB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-136729OB-I00
  • dc.rights © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Adherens junctions
  • dc.subject.keyword Electron microscopy
  • dc.title Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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