Regulation of the Na+/K+-ATPase Ena1 expression by calcineurin/Crz1 under high pH stress: a quantitative study

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  • dc.contributor.author Petrezsélyová, Silvia
  • dc.contributor.author López-Malo, María
  • dc.contributor.author Canadell, David
  • dc.contributor.author Roque, Alicia
  • dc.contributor.author Serra-Cardona, Albert
  • dc.contributor.author Marqués, M. Carmen
  • dc.contributor.author Vilaprinyó, Ester
  • dc.contributor.author Alves, Rui
  • dc.contributor.author Yenush, Lynne
  • dc.contributor.author Ariño, Joaquín
  • dc.date.accessioned 2024-10-22T06:31:43Z
  • dc.date.available 2024-10-22T06:31:43Z
  • dc.date.issued 2016
  • dc.description.abstract Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response. Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1-binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%. The models suggest the existence of a second input with similar kinetics, which would be likely mediated by high pH-induced activation of the Snf1 kinase.
  • dc.description.sponsorship This work was supported by grants BFU2011-30197-C3-01, BFU2014-54591-C2-1-P and EUI2009-04147 (SysMo2) to JA. (Ministry of Industry and Competitivity, Spain, and Fondo Europeo de Desarrollo Regional [FEDER]). JA is the recipient of an Ajut 2014SGR-4 award (Generalitat de Catalunya). DC was recipient of a predoctoral fellowship from the Spanish Ministry of Education. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Petrezsélyová S, López-Malo M, Canadell D, Roque A, Serra-Cardona A, Marqués MC, et al. Regulation of the Na+/K+-ATPase Ena1 expression by calcineurin/Crz1 under high pH stress: a quantitative study. PLoS ONE. 2016 Jun 30;11(6):e0158424. DOI: 10.1371/journal.pone.0158424
  • dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0158424
  • dc.identifier.issn 1932-6203
  • dc.identifier.uri http://hdl.handle.net/10230/68297
  • dc.language.iso eng
  • dc.publisher Public Library of Science (PLoS)
  • dc.relation.ispartof PLoS ONE. 2016 Jun 30;11(6):e0158424
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-30197-C3-01
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-54591-C2-1-P
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/EUI2009-04147
  • dc.rights © 2016 Petrezsélyová et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.other Llevats
  • dc.subject.other Cations
  • dc.subject.other Investigació quantitativa
  • dc.title Regulation of the Na+/K+-ATPase Ena1 expression by calcineurin/Crz1 under high pH stress: a quantitative study
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion