Passive stiffness of left ventricular myocardial tissue is reduced by ovariectomy in a post-menopause mouse model

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• dc.contributor.author Farré López, Núria
• dc.contributor.author Jorba, Ignasi
• dc.contributor.author Torres, Marta
• dc.contributor.author Falcones, Bryan
• dc.contributor.author Martí Almor, Julio
• dc.contributor.author Farré, Ramon
• dc.contributor.author Almendros, Isaac
• dc.contributor.author Navajas, Daniel
• dc.date.accessioned 2019-04-30T07:49:44Z
• dc.date.available 2019-04-30T07:49:44Z
• dc.date.issued 2018
• dc.description.abstract Background: Heart failure (HF) - a very prevalent disease with high morbidity and mortality - usually presents with diastolic dysfunction. Although post-menopause women are at increased risk of HF and diastolic dysfunction, poor attention has been paid to clinically and experimentally investigate this group of patients. Specifically, whether myocardial stiffness is affected by menopause is unknown. Aim: To investigate whether loss of female sexual hormones modifies the Young's modulus (E) of left ventricular (LV) myocardial tissue in a mouse model of menopause induced by ovariectomy (OVX). Methods: After 6 months of bilateral OVX, eight mice were sacrificed, fresh LV myocardial strips were prepared (∼8 × 1 × 1 mm), and their passive stress-stretch relationship was measured. E was computed by exponential fitting of the stress-stretch relationship. Subsequently, to assess the relative role of cellular and extracellular matrix components in determining OVX-induced changes in E, the tissues strips were decellularized and subjected to the same stretching protocol to measure E. A control group of eight sham-OVX mice was simultaneously studied. Results: E (kPa; m ± SE) in OVX mice was ∼twofold lower than in controls (11.7 ± 1.8 and 22.1 ± 4.4, respectively; p < 0.05). No significant difference between groups was found in E of the decellularized tissue (31.4 ± 12.05 and 40.9 ± 11.5, respectively; p = 0.58). Conclusion: Loss of female sexual hormones in an OVX model induces a reduction in the passive stiffness of myocardial tissue, suggesting that active relaxation should play a counterbalancing role in diastolic dysfunction in post-menopausal women with HF.
• dc.description.sponsorship This work was supported in part by the Spanish Ministry of Economy and Competitiveness (Grant Nos. FIS-PI14/00280, SAF2017-85574-R, and DPI2017-83721-P) and Generalitat de Catalunya (CERCA Program).
• dc.format.mimetype application/pdf
• dc.identifier.citation Farré N, Jorba I, Torres M, Falcones B, Martí-Almor J, Farré R. et al. Passive stiffness of left ventricular myocardial tissue is reduced by ovariectomy in a post-menopause mouse model. Front Physiol. 2018 Nov 5;9:1545. DOI: 10.3389/fphys.2018.01545
• dc.identifier.doi http://dx.doi.org/10.3389/fphys.2018.01545
• dc.identifier.issn 1664-042X
• dc.identifier.uri http://hdl.handle.net/10230/37157
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Frontiers in Physiology. 2018 Nov 5;9:1545
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/SAF2017-85574-R
• dc.rights Copyright © 2018 Farré, Jorba, Torres, Falcones, Martí-Almor, Farré, Almendros and Navajas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Decellularized tissue
• dc.subject.keyword Female hormones
• dc.subject.keyword Heart tissue
• dc.subject.keyword Ovariectomy
• dc.subject.keyword Stress-strain
• dc.title Passive stiffness of left ventricular myocardial tissue is reduced by ovariectomy in a post-menopause mouse model
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion