Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Coll, Olga
• dc.contributor.author Guitart, Tanit
• dc.contributor.author Villalba, Ana
• dc.contributor.author Papin, Catherine
• dc.contributor.author Simonelig, Martine
• dc.contributor.author Gebauer, Fátima
• dc.date.accessioned 2019-12-05T08:38:03Z
• dc.date.available 2019-12-05T08:38:03Z
• dc.date.issued 2018
• dc.description.abstract Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation. In vertebrates, this process requires two sequence elements in target 3' UTRs: the U-rich cytoplasmic polyadenylation element and the AAUAAA hexanucleotide. In Drosophila melanogaster, cytoplasmic polyadenylation of Toll mRNA occurs independently of these canonical elements and requires a machinery that remains to be characterized. Here we identify Dicer-2 as a component of this machinery. Dicer-2, a factor previously involved in RNA interference (RNAi), interacts with the cytoplasmic poly(A) polymerase Wispy. Depletion of Dicer-2 from polyadenylation-competent embryo extracts and analysis of wispy mutants indicate that both factors are necessary for polyadenylation and translation of Toll mRNA. We further identify r2d2 mRNA, encoding a Dicer-2 partner in RNAi, as a Dicer-2 polyadenylation target. Our results uncover a novel function of Dicer-2 in activation of mRNA translation through cytoplasmic polyadenylation.
• dc.description.sponsorship This work was supported by MINECO and the European Regional Development Fund (ERDF) under BFU2012-37135, BFU2015-68741, and Consolider CSD2009-00080 grants to F.G., and by the CNRS UMR9002, ANR (ANR-2010-BLAN-1201-01 and ANR-15-CE12-0019-01), and FRM (Equipe FRM 2013 DEQ20130326534) to M.S. We acknowledge support of MINECO “Centro de Excelencia Severo Ochoa 2013-2017,” SEV-2012-0208.
• dc.format.mimetype application/pdf
• dc.identifier.citation Coll O, Guitart T, Villalba A, Papin C, Simonelig M, Gebauer F. Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation. RNA. 2018;24(4):529-39. DOI: 10.1261/rna.065417.117
• dc.identifier.doi http://dx.doi.org/10.1261/rna.065417.117
• dc.identifier.issn 1355-8382
• dc.identifier.uri http://hdl.handle.net/10230/43101
• dc.language.iso eng
• dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)
• dc.relation.ispartof RNA. 2018;24(4):529-39
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-37135
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68741
• dc.rights © 2018 Coll et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Dicer-2
• dc.subject.keyword Wispy
• dc.subject.keyword Cytoplasmic polyadenylation
• dc.subject.keyword Translation
• dc.title Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion