CSDE1 controls gene expression through the miRNA-mediated decay machinery

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  • dc.contributor.author Kakumani, Pavan Kumar
  • dc.contributor.author Harvey, Louis-Mathieu
  • dc.contributor.author Houle, François
  • dc.contributor.author Guitart, Tanit
  • dc.contributor.author Gebauer, Fátima
  • dc.contributor.author Simard, Martin J.
  • dc.date.accessioned 2020-05-12T07:09:55Z
  • dc.date.available 2020-05-12T07:09:55Z
  • dc.date.issued 2020
  • dc.description.abstract In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1-DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway.
  • dc.description.sponsorship We also thank all members of our laboratory for helpful discussions and the mass spectrometry facility from the Centre Hospitalier Universitaire de Québec-Université Laval Research Center. Funding: The Canadian Institutes of Health Research supported this work (MJ Simard). PK Kakumani and LM Harvey are recipients of scholarship from the Fonds de Recherche du Québec-Santé (FRQ-S). MJ Simard is a Research Chair from FRQ-S. F Gebauer was supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015-68741), the Catalan Government (2017 SGR 799), Marató de TV3 (TV′13-20131430), and the Centre of Excellence Severo Ochoa.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Kakumani PK, Harvey LM, Houle F, Guitart T, Gebauer F, Simard MJ. CSDE1 controls gene expression through the miRNA-mediated decay machinery. Life Sci Alliance. 2020; 3(4). pii: e201900632. DOI: 10.26508/lsa.201900632
  • dc.identifier.doi http://dx.doi.org/10.26508/lsa.201900632
  • dc.identifier.issn 2575-1077
  • dc.identifier.uri http://hdl.handle.net/10230/44489
  • dc.language.iso eng
  • dc.publisher Life Science Alliance
  • dc.relation.ispartof Life Sci Alliance. 2020; 3(4). pii: e201900632
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68741
  • dc.rights © 2020 Kakumani et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.title CSDE1 controls gene expression through the miRNA-mediated decay machinery
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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