Automated cardiac sarcomere analysis from second harmonic generation images

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author García Cañadilla, Patricia, 1985-ca
  • dc.contributor.author Gonzalez-Tendero, Annaca
  • dc.contributor.author Iruretagoyena, Igorca
  • dc.contributor.author Crispi Brillas, Fàtimaca
  • dc.contributor.author Torre, Iratxeca
  • dc.contributor.author Amat-Roldán, Ivánca
  • dc.contributor.author Bijnens, Bartca
  • dc.contributor.author Gratacós Solsona, Eduardca
  • dc.date.accessioned 2017-03-09T09:35:59Z
  • dc.date.available 2017-03-09T09:35:59Z
  • dc.date.issued 2014
  • dc.description.abstract Automatic quantification of cardiac muscle properties in tissue sections might provide important information related to different types of diseases. Second harmonic generation (SHG) imaging provides a stain-free microscopy approach to image cardiac fibers that, combined with our methodology of the automated measurement of the ultrastructure of muscle fibers, computes a reliable set of quantitative image features (sarcomere length, A-band length, thick–thin interaction length, and fiber orientation). We evaluated the performance of our methodology in computer-generated muscle fibers modeling some artifacts that are present during the image acquisition. Then, we also evaluated it by comparing it to manual measurements in SHG images from cardiac tissue of fetal and adult rabbits. The results showed a good performance of our methodology at high signal-to-noise ratio of 20 dB. We conclude that our automated measurements enable reliable characterization of cardiac fiber tissues to systematically study cardiac tissue in a wide range of conditions.en
  • dc.description.sponsorship The authors acknowledge the cvRemod project under the CENIT 20092012 program of the Industrial and Technological Development Center (CDTI). This study was partially supported by grants from Instituto de Salud Carlos III and Ministerio de Economia y Competitividad (Ref. PI11/00051, PI11/01709, PI12/00801, and SAF2012-37196), Fondo Europeo de Desarrollo Regional de la Unión Europea “Una manera de hacer Europa,” Spain; Obra Social ‘La Caixa,’ Spain; the Fundación Mutua Madrileña; Fundació Agrupació Mutua; Cerebra Foundation for the Brain Injured Child (Carmarthen, Wales, UK); and the Seventh Framework Programme (FP7/2007-2013) under Grant No. 611823. P.G.C. was supported by the Programa de Ayudas Predoctorales de Formación en investigación en Salud (FI12/00362) from the Instituto Carlos III, and A.G.T. was supported by IDIBAPS Predoctoral Programme.en
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Garcia-Canadilla P, Gonzalez-Tendero A, Iruretagoyena I, Crispi F, Torre I, Amat-Roldan I, Bijnens B, Gratacos E. Automated cardiac sarcomere analysis from second harmonic generation images. J Biomed Opt. 2014;19(5):056010. DOI: 10.1117/1.JBO.19.5.056010
  • dc.identifier.doi http://dx.doi.org/10.1117/1.JBO.19.5.056010
  • dc.identifier.issn 1083-3668
  • dc.identifier.uri http://hdl.handle.net/10230/28192
  • dc.language.iso eng
  • dc.publisher Society of Photo Optical Instrumentation Engineers (SPIE)ca
  • dc.relation.ispartof J Biomed Opt. 2014;19(5):056010.
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/611823
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2012-37196
  • dc.rights © (2014) Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Garcia-Canadilla P, Gonzalez-Tendero A, Iruretagoyena I, Crispi F, Torre I, Amat-Roldan I, Bijnens B, Gratacos E. Automated cardiac sarcomere analysis from second harmonic generation images. J Biomed Opt. 2014;19(5):056010. http://dx.doi.org/10.1117/1.JBO.19.5.056010
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.subject.keyword Second-harmonic generationen
  • dc.subject.keyword Sarcomere lengthen
  • dc.subject.keyword Autocorrelationen
  • dc.subject.keyword Fiber orientationen
  • dc.title Automated cardiac sarcomere analysis from second harmonic generation imagesca
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Departament de Tecnologies de la Informació i les Comunicacions)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)