Genome comparisons of Candida glabrata serial clinical isolates reveal patterns of genetic variation in infecting clonal populations

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Carreté Muñoz, Laia, 1990-
  • dc.contributor.author Ksiezopolska, Ewa
  • dc.contributor.author Gómez-Molero, Emilia
  • dc.contributor.author Angoulvant, Adela
  • dc.contributor.author Bader, Olivier
  • dc.contributor.author Fairhead, Cécile
  • dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
  • dc.date.accessioned 2019-03-05T08:13:18Z
  • dc.date.available 2019-03-05T08:13:18Z
  • dc.date.issued 2019
  • dc.description.abstract Candida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although the mechanisms underlying virulence and drug resistance in C. glabrata are now starting to be elucidated, we still lack a good understanding of how this yeast adapts during the course of an infection. Outstanding questions are whether the observed genomic plasticity of C. glabrata plays a role during infection, or what levels of genetic variation exist within an infecting clonal population. To shed light onto the genomic variation within infecting C. glabrata populations, we compared the genomes of 11 pairs and one trio of serial clinical isolates, each obtained from a single patient. Our results provide a catalog of genetic variations existing within clonal infecting isolates, and reveal an enrichment of non-synonymous changes in genes encoding cell-wall proteins. Genetic variation and the presence of non-synonymous mutations and copy number variations accumulated within the host, suggest that clonal populations entail a non-negligible level of genetic variation that may reflect selection processes that occur within the human body. As we show here, these genomic changes can underlie phenotypic differences in traits that are relevant for infection.
  • dc.description.sponsorship TG group acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness (MEIC) for the EMBL partnership, and grants “Centro de Excelencia Severo Ochoa 2013–2017” SEV-2012-0208, and BFU2015-67107 cofunded by European Regional Development Fund (ERDF), from the CERCA Programme/Generalitat de Catalunya; from the Catalan Research Agency (AGAUR) SGR857, and grant from the European Union’s Horizon 2020 research and innovation programme under the grant agreement ERC-2016-724173 the Marie Sklodowska-Curie grant agreement No. H2020-MSCA-ITN-2014-642095. This project received support from the INB Grant (PT17/0009/0023 – ISCIII-SGEFI/ERDF).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Carreté L, Ksiezopolska E, Gómez-Molero E, Angoulvant A, Bader O, Fairhead C et al. Genome comparisons of Candida glabrata serial clinical isolates reveal patterns of genetic variation in infecting clonal populations. Front Microbiol. 2019;10:112. DOI: 10.3389/fmicb.2019.00112
  • dc.identifier.doi http://dx.doi.org/10.3389/fmicb.2019.00112
  • dc.identifier.issn 1664-302X
  • dc.identifier.uri http://hdl.handle.net/10230/36730
  • dc.language.iso eng
  • dc.publisher Frontiers
  • dc.relation.ispartof Frontiers in Microbiology. 2019;10:112
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-67107
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/724173
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/642095
  • dc.rights © 2019 Carreté, Ksiezopolska, Gómez-Molero, Angoulvant, Bader, Fairhead and Gabaldón. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Candidiasis
  • dc.subject.keyword Candida glabrata
  • dc.subject.keyword Clinical isolates
  • dc.subject.keyword Resistance
  • dc.subject.keyword Genome sequencing
  • dc.subject.keyword Genome variation
  • dc.title Genome comparisons of Candida glabrata serial clinical isolates reveal patterns of genetic variation in infecting clonal populations
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Center for Genomic Regulation (CRG))
Articles (Departament de Medicina i Ciències de la Vida)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)