Budding yeast complete DNA synthesis after chromosome segregation begins

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• dc.contributor.author Ivanova, Tsvetomira Georgieva, 1978-
• dc.contributor.author Maier, Michael, 1983-
• dc.contributor.author Missarova, Alsu, 1990-
• dc.contributor.author Ziegler-Birling, Céline
• dc.contributor.author Dam, Monica
• dc.contributor.author Gomar-Alba, Mercè
• dc.contributor.author Carey, Lucas, 1980-
• dc.contributor.author Mendoza, Manuel (Mendoza Palomares)
• dc.date.accessioned 2020-06-04T06:23:21Z
• dc.date.available 2020-06-04T06:23:21Z
• dc.date.issued 2020
• dc.description.abstract To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that in 20-40% of unperturbed yeast cells, DNA synthesis continues during anaphase, late in mitosis. High cyclin-Cdk activity inhibits DNA synthesis in metaphase, and the decrease in cyclin-Cdk activity during mitotic exit allows DNA synthesis to finish at subtelomeric and some difficult-to-replicate regions. DNA synthesis during late mitosis correlates with elevated mutation rates at subtelomeric regions, including copy number variation. Thus, yeast cells temporally overlap DNA synthesis and chromosome segregation during normal growth, possibly allowing cells to maximize population-level growth rate while simultaneously exploring greater genetic space.
• dc.description.sponsorship This study was supported by Ministerio de Economía y Competitividad (MINECO) (BFU2015-68351-P) and AGAUR (2014SGR0974 & 2017SGR1054) grants to L.B.C. and the Unidad de Excelencia María de Maeztu, funded by the MINECO (MDM-2014-0370); the European Research Council (ERC) Starting Grant 2010-St-20091118 to Ma.M., the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013–2017’, SEV- 2012-0208 to the CRG and the grant ANR-10-LABX-0030-INRT, which is a French State fund managed by the Agence Nationale de la Recherche under the frame programme Investissements d’Avenir ANR-10-IDEX-0002-02 to the IGBMC.
• dc.format.mimetype application/pdf
• dc.identifier.citation Ivanova T, Maier M, Missarova A, Ziegler-Birling C, Dam M, Gomar-Alba M, Carey LB, Mendoza M. Budding yeast complete DNA synthesis after chromosome segregation begins. Nat Commun. 2020; 11(1):2267. DOI: 10.1038/s41467-020-16100-3
• dc.identifier.doi http://dx.doi.org/10.1038/s41467-020-16100-3
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/44893
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Nat Commun. 2020; 11(1):2267
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68351-P
• dc.rights © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Chromosome segregation
• dc.subject.keyword DNA synthesis
• dc.title Budding yeast complete DNA synthesis after chromosome segregation begins
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion