Timing of gene expression in a cell-fate decision system

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• dc.contributor.author Aymoz, Delphine
• dc.contributor.author Solé, Carme
• dc.contributor.author Pierre, Jean-Jerrold
• dc.contributor.author Schmitt, Marta
• dc.contributor.author Nadal Clanchet, Eulàlia de
• dc.contributor.author Posas Garriga, Francesc
• dc.contributor.author Pelet, Serge
• dc.date.accessioned 2018-12-20T15:34:45Z
• dc.date.available 2018-12-20T15:34:45Z
• dc.date.issued 2018
• dc.description.abstract During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the physiological context of mating. In both conditions, we observed striking differences in the timing of induction of mating-responsive promoters. Biochemical analyses and generation of synthetic promoter variants demonstrated how the interplay between transcription factor binding and nucleosomes contributes to determine the kinetics of transcription in a simplified cell-fate decision system.
• dc.description.sponsorship Work in the Pelet laboratory is supported by the Swiss National Science Foundation (PP00P3_139121), SystemsX.ch, and the University of Lausanne. Work in the Posas and de Nadal laboratories is funded by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015‐64437‐P and FEDER to FP; BFU2017‐85152‐P and FEDER to EN), the Catalan Government (2017 SGR 799), the Fundación Botín, and the Banco Santander through its Santander Universities Global Division to FP. FP is recipient of an ICREA Acadèmia (Generalitat de Catalunya).
• dc.format.mimetype application/pdf
• dc.identifier.citation Aymoz D, Solé C, Pierre JJ, Schmitt M, de Nadal E, Posas F, Pelet S. Timing of gene expression in a cell-fate decision system. Mol Syst Biol. 2018;14(4):e8024. DOI: 10.15252/msb.20178024
• dc.identifier.doi http://dx.doi.org/10.15252/msb.20178024
• dc.identifier.issn 1744-4292
• dc.identifier.uri http://hdl.handle.net/10230/36165
• dc.language.iso eng
• dc.publisher Wiley-Blackwell
• dc.relation.ispartof Molecular Systems Biology. 2018;14(4):e8024
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015‐64437‐P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2017‐85152‐P
• dc.rights © 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Gene expression
• dc.subject.keyword MAPK pathway
• dc.subject.keyword Single‐cell measurements
• dc.subject.keyword Yeast mating
• dc.title Timing of gene expression in a cell-fate decision system
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion