The Hsp40 Mas5 connects protein quality control and the general stress response through the thermo-sensitive Pyp1

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• dc.contributor.author Boronat i Llop, Susanna, 1965-
• dc.contributor.author Marte, Luis, 1990-
• dc.contributor.author Vega, Montserrat
• dc.contributor.author García Santamarina, Sarela, 1978-
• dc.contributor.author Cabrera, Margarita
• dc.contributor.author Ayté del Olmo, José
• dc.contributor.author Hidalgo Hernando, Elena
• dc.date.accessioned 2021-01-13T07:14:35Z
• dc.date.available 2021-01-13T07:14:35Z
• dc.date.issued 2020
• dc.description.abstract Upon heat shock, the fission yeast Hsp40 chaperone Mas5 drives temperature-sensitive proteins toward protein aggregate centers (PACs) to avoid their degradation until lower temperatures favor their refolding. We show here that cells lacking Mas5 are resistant to oxidative stress. Components of the general stress pathways, the MAP kinase Sty1 and the transcription factor Atf1, are suppressors of this phenotype. Strain Δmas5 expresses higher levels of Sty1- and Atf1-dependent stress genes than wild-type cells. Pyp1, the main tyrosine phosphatase maintaining Sty1 inactive in the absence of stress, is a temperature-sensitive protein that aggregates upon temperature up-shifts in a Mas5-dependent manner. In strain Δmas5, Pyp1 is sent to proteasomal degradation even in the absence of stress. We propose that Pyp1 is a thermo-sensitive phosphatase, which during heat stress coalescences into PACs in a Mas5-dependent manner, to promote full activation of the anti-stress Sty1-Atf1 cascade.
• dc.description.sponsorship This work is supported by the Ministerio de Ciencia, Innovación y Universidades, PLAN E and FEDER (Spain) (PGC2018-093920-B-I00 to E.H.). The Oxidative Stress and Cell Cycle group is also supported by Generalitat de Catalunya (Spain) (2017-SGR-539) and by Unidad de Excelencia María de Maeztu, funded by the AEI (Spain) (CEX2018-000792-M). M.C. is funded by the Ramon y Cajal program (Spain) (MINECO-RYC2013-12858). E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain).
• dc.format.mimetype application/pdf
• dc.identifier.citation Boronat S, Marte L, Vega M, García-Santamarina S, Cabrera M, Ayté J, Hidalgo E. The Hsp40 Mas5 connects protein quality control and the general stress response through the thermo-sensitive Pyp1. iScience. 2020; 23(11):101725. DOI: 10.1016/j.isci.2020.101725
• dc.identifier.doi http://dx.doi.org/10.1016/j.isci.2020.101725
• dc.identifier.issn 2589-0042
• dc.identifier.uri http://hdl.handle.net/10230/46151
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof iScience. 2020; 23(11):101725
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00
• dc.rights © 2020 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword Biological sciences
• dc.subject.keyword Cell biology
• dc.subject.keyword Molecular biology
• dc.title The Hsp40 Mas5 connects protein quality control and the general stress response through the thermo-sensitive Pyp1
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion