A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle
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- dc.contributor.author Dejea i Velardo, Hèctor
- dc.contributor.author Schlepütz, Christian M.
- dc.contributor.author Méndez-Carmona, Natalia
- dc.contributor.author Arnold, Maria
- dc.contributor.author García Cañadilla, Patricia, 1985-
- dc.contributor.author Longnus, Sarah L.
- dc.contributor.author Stampanoni, Marco
- dc.contributor.author Bijnens, Bart
- dc.contributor.author Bonnin, Anne
- dc.date.accessioned 2025-10-20T06:19:39Z
- dc.date.available 2025-10-20T06:19:39Z
- dc.date.issued 2022
- dc.description.abstract Introduction: Cardiac architecture has been extensively investigated ex vivo using a broad spectrum of imaging techniques. Nevertheless, the heart is a dynamic system and the structural mechanisms governing the cardiac cycle can only be unveiled when investigating it as such. Methods: This work presents the customization of an isolated, perfused heart system compatible with synchrotron-based X-ray phase contrast imaging (X-PCI). Results: Thanks to the capabilities of the developed setup, it was possible to visualize a beating isolated, perfused rat heart for the very first time in 4D at an unprecedented 2.75 μm pixel size (10.6 μm spatial resolution), and 1 ms temporal resolution. Discussion: The customized setup allows high-spatial resolution studies of heart architecture along the cardiac cycle and has thus the potential to serve as a tool for the characterization of the structural dynamics of the heart, including the effects of drugs and other substances able to modify the cardiac cycle.en
- dc.description.sponsorship This project was supported by the Grant No. #2017-303 of the Strategic Focal Area Personalized Health and Related Technologies (PHRT) of the ETH Domain, the Spanish Ministry of Economy and Competitiveness (Grant No. TIN2014-52923-R) and FEDER. Open access funding provided by PSI (Paul Scherrer Institute).en
- dc.format.mimetype application/pdf
- dc.identifier.citation Dejea H, Schlepütz CM, Méndez-Carmona N, Arnold M, Garcia-Canadilla P, Longnus SL, et al. A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle. Front Cardiovasc Med. 2022 Dec 22;9:1023483. DOI: 10.3389/fcvm.2022.1023483
- dc.identifier.doi http://dx.doi.org/10.3389/fcvm.2022.1023483
- dc.identifier.issn 2297-055X
- dc.identifier.uri http://hdl.handle.net/10230/71556
- dc.language.iso eng
- dc.publisher Frontiers
- dc.relation.ispartof Frontiers in Cardiovascular Medicine. 2022 Dec 22;9:1023483
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TIN2014-52923-R
- dc.rights © 2022 Dejea, Schlepütz, Méndez-Carmona, Arnold, Garcia-Canadilla, Longnus, Stampanoni, Bijnens and Bonnin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Synchrotronen
- dc.subject.keyword Tomographic microscopyen
- dc.subject.keyword Langendorffen
- dc.subject.keyword 4D imagingen
- dc.subject.keyword Cardiac cycleen
- dc.title A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycleen
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion