Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
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- dc.contributor.author Olarte, Omar E.ca
- dc.contributor.author Licea-Rodriguez, Jacobca
- dc.contributor.author Palero, Jonathan A.ca
- dc.contributor.author Gualda, Emilio J.ca
- dc.contributor.author Artigas, Davidca
- dc.contributor.author Mayer, Jürgen, 1977-ca
- dc.contributor.author Swoger, Jimca
- dc.contributor.author Sharpe, Jamesca
- dc.contributor.author Rocha-Mendoza, Israelca
- dc.contributor.author Rangel-Rojo, Raulca
- dc.contributor.author Loza-Alvarez, Pabloca
- dc.date.accessioned 2018-07-19T07:33:24Z
- dc.date.available 2018-07-19T07:33:24Z
- dc.date.issued 2012
- dc.description.abstract We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivoCaenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.
- dc.description.sponsorship This work is supported by the Generalitat de Catalunya grant 2009-SGR-159, the Spanish government grants TEC2009-09698 and SAF2008-00211, the EU project STELUM (FP7-PEOPLE-2007-3-1-IAPP, 217997), the Swiss National Science Foundation (SNSF) Sinergia project grant CRSII3_125447, the FP7-NMP VIBRANT project grant CP-IP 228933-2, the NoE P4L and Laserlab optobio. Jacob Licea-Rodriguez acknowledges CONACYT Mexico for supporting his stay at ICFO through a mixed fellowship. This research has been partially supported by Fundació Cellex Barcelona and was mainly conducted at ICFO’s “Super-Resolution Light Microscopy and Nanoscopy Facility”
- dc.format.mimetype application/pdf
- dc.identifier.citation Olarte OE, Licea-Rodriguez J, Palero JA, Gualda EJ, Artigas D, Mayer J et al. Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles. Biomed Opt Express. 2012 Jul 1;3(7):1492-505. DOI: 10.1364/BOE.3.001492
- dc.identifier.doi http://dx.doi.org/10.1364/BOE.3.001492
- dc.identifier.issn 2156-7085
- dc.identifier.uri http://hdl.handle.net/10230/35196
- dc.language.iso eng
- dc.publisher Optical Society of America (OSA)ca
- dc.relation.ispartof Biomedical Optics Express. 2012 Jul 1;3(7):1492-505
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/TEC2009-09698
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2008-00211
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/217997
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/228933
- dc.rights © Oman E. Olarte et al 2012 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.other Feixos de làser
- dc.subject.other Microscòpia de fluorescència
- dc.subject.other Microscòpia
- dc.subject.other Processos multifotó
- dc.title Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profilesca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion