Gene expression of desaturase (FADS1 and FADS2) and elongase (ELOVL5) enzymes in peripheral blood: association with polyunsaturated fatty acid levels and atopic eczema in 4-year-old children

Chisaguano, Aida M.; Montes, Rosa; Pérez Berezo, Teresa; Castellote, Ana Isabel; Guerendiain, Marcela; Bustamante Pineda, Mariona; Morales, Eva; García Esteban, Raquel; Sunyer Deu, Jordi; Franch, Àngels; López Sabater, M. Carmen

Gene expression of desaturase (FADS1 and FADS2) and elongase (ELOVL5) enzymes in peripheral blood: association with polyunsaturated fatty acid levels and atopic eczema in 4-year-old children

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dc.contributor.author Chisaguano, Aida M.ca
dc.contributor.author Montes, Rosaca
dc.contributor.author Pérez Berezo, Teresaca
dc.contributor.author Castellote, Ana Isabelca
dc.contributor.author Guerendiain, Marcelaca
dc.contributor.author Bustamante Pineda, Marionaca
dc.contributor.author Morales, Evaca
dc.contributor.author García Esteban, Raquelca
dc.contributor.author Sunyer Deu, Jordica
dc.contributor.author Franch, Àngelsca
dc.contributor.author López Sabater, M. Carmenca
dc.date.accessioned 2015-05-15T07:10:27Z
dc.date.available 2015-05-15T07:10:27Z
dc.date.issued 2013ca
dc.description.abstract Background: It is unknown if changes in the gene expression of the desaturase and elongase enzymes are associated with abnormal n-6 long chain polyunsaturated fatty acid (LC-PUFA) levels in children with atopic eczema (AE). We analyzed whether mRNA-expression of genes encoding key enzymes of LC-PUFA synthesis (FADS1, FADS2 and ELOVL5) is associated with circulating LC-PUFA levels and risk of AE in 4-year-old children. Methods: AE (n=20) and non-AE (n=104) children participating in the Sabadell cohort within the INfancia y Medio Ambiente (INMA) Project were included in the present study. RT-PCR with TaqMan Low-Density Array cards was used to measure the mRNA-expression of FADS1, FADS2 and ELOVL5. LC-PUFA levels were measured by fast gas chromatography in plasma phospholipids. The relationship of gene expression with LC-PUFA levels and enzyme activities was evaluated by Pearson’s rank correlation coefficient, and logistic regression models were used to study its association with risk of developing AE. Results: Children with AE had lower levels of several n-6 PUFA members, dihomo-γ-linolenic (DGLA) and arachidonic (AA) acids. mRNA-expression levels of FADS1 and 2 strongly correlated with DGLA levels and with D6D activity. FADS2 and ELOVL5 mRNA-expression levels were significantly lower in AE than in non-AE children (-40.30% and -20.36%; respectively), but no differences were found for FADS1. Conclusions and Significance: Changes in the mRNA-expression levels of FADS1 and 2 directly affect blood DGLA levels and D6D activity. This study suggests that lower mRNA-expressions of FADS2 and ELOVL5 are associated with higher risk of atopic eczema in young children.en
dc.description.sponsorship The following sources of funding supported the work: The Spanish Ministry of Science and Innovation for the financial support given to the research project AGL2009-09730/ALI. www.micinn.es. The Spanish Ministry of Economy and Competitiveness for the financial support given to the research project BUF2012-40254-C03-02. www.mineco.gob.esen
dc.format.mimetype application/pdfca
dc.identifier.citation Chisaguano AM, Montes R, Pérez-Berezo T, Castellote AI, Guerendiain M, Bustamante M et al. Gene expression of desaturase (FADS1 and FADS2) and elongase (ELOVL5) enzymes in peripheral blood: association with polyunsaturated fatty acid levels and atopic eczema in 4-year-old children. PLoS ONE. 2013;8(10):e78245. DOI: 10.1371/journal.pone.0078245ca
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0078245
dc.identifier.issn 1932-6203ca
dc.identifier.uri http://hdl.handle.net/10230/23585
dc.language.iso engca
dc.publisher Public Library of Science (PLoS)ca
dc.relation.ispartof PLoS ONE. 2013;8(10):e78245
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/AGL2009-09730
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BUF2012-40254
dc.rights © 2013 Chisaguano et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.subject.other RNA missatgerca
dc.subject.other Regulació genèticaca
dc.subject.other Àcids grassosca
dc.title Gene expression of desaturase (FADS1 and FADS2) and elongase (ELOVL5) enzymes in peripheral blood: association with polyunsaturated fatty acid levels and atopic eczema in 4-year-old childrenen
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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