omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells

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• dc.contributor.author Granja, Ricardoca
• dc.contributor.author Fernández-Fernández, José Manuel, 1967-ca
• dc.contributor.author Izaguirre, Victorca
• dc.contributor.author González García, Carmenca
• dc.contributor.author Ceña, Valentinca
• dc.date.accessioned 2016-01-21T19:00:05Z
• dc.date.available 2016-01-21T19:00:05Z
• dc.date.issued 1995
• dc.description.abstract We have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using omega-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that omega-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by omega-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, omega-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.ca
• dc.description.sponsorship This work was supported, in part, by grants from Fundación Salud 2000, Dirección General de Investigación Cientifica y Técnica, Grant PM92-0112, and the European Economic Community, Grant SC1"-CT91-0709. R.G. was supported by a University of Alicante fellowship, J.M.F. by a CAM fellowship, and V.I. by a Generalitat Valenciana fellowship.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Granja R, Fernández-Fernández JM, Izaguirre V, González-García C, Ceña V. omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells. FEBS letters. 1995;362(1):15-8. DOI: 10.1016/0014-5793(95)00149-4ca
• dc.identifier.doi http://dx.doi.org/10.1016/0014-5793(95)00149-4
• dc.identifier.issn 0014-5793
• dc.identifier.uri http://hdl.handle.net/10230/25632
• dc.language.iso engca
• dc.publisher Elsevierca
• dc.relation.ispartof FEBS letters. 1995;362(1):15-8
• dc.rights © Elsevier This is the published version of an article http://dx.doi.org/10.1016/0014-5793(95)00149-4 that appeared in the journal Developmental cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword Calcium channel
• dc.subject.keyword P-type
• dc.subject.keyword Nicotinic receptor
• dc.subject.keyword omega-Agatoxin IVA
• dc.subject.keyword Secretion
• dc.subject.keyword Chromaffin cell
• dc.subject.other Canals de potassica
• dc.subject.other Nicotinaca
• dc.title omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cellsca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca