omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells

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dc.contributor.authorGranja, Ricardoca
dc.contributor.authorFernández-Fernández, José Manuel, 1967-ca
dc.contributor.authorIzaguirre, Victorca
dc.contributor.authorGonzález García, Carmenca
dc.contributor.authorCeña, Valentinca
dc.date.accessioned2016-01-21T19:00:05Z
dc.date.available2016-01-21T19:00:05Z
dc.date.issued1995
dc.description.abstractWe have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using omega-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that omega-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by omega-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, omega-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.ca
dc.description.sponsorshipThis work was supported, in part, by grants from Fundación Salud 2000, Dirección General de Investigación Cientifica y Técnica, Grant PM92-0112, and the European Economic Community, Grant SC1"-CT91-0709. R.G. was supported by a University of Alicante fellowship, J.M.F. by a CAM fellowship, and V.I. by a Generalitat Valenciana fellowship.
dc.format.mimetypeapplication/pdfca
dc.identifier.citationGranja R, Fernández-Fernández JM, Izaguirre V, González-García C, Ceña V. omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells. FEBS letters. 1995;362(1):15-8. DOI: 10.1016/0014-5793(95)00149-4ca
dc.identifier.doihttp://dx.doi.org/10.1016/0014-5793(95)00149-4
dc.identifier.issn0014-5793
dc.identifier.urihttp://hdl.handle.net/10230/25632
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofFEBS letters. 1995;362(1):15-8
dc.rights© Elsevier This is the published version of an article http://dx.doi.org/10.1016/0014-5793(95)00149-4 that appeared in the journal Developmental cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseca
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca
dc.subject.keywordCalcium channel
dc.subject.keywordP-type
dc.subject.keywordNicotinic receptor
dc.subject.keywordomega-Agatoxin IVA
dc.subject.keywordSecretion
dc.subject.keywordChromaffin cell
dc.subject.otherCanals de potassica
dc.subject.otherNicotinaca
dc.titleomega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cellsca
dc.typeinfo:eu-repo/semantics/articleca
dc.type.versioninfo:eu-repo/semantics/publishedVersionca

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