A new variant of the aadE-sat4-aphA-3 gene cluster found in a conjugative plasmid from a MDR campylobacter jejuni isolate

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• dc.contributor.author Guirado, Pedro
• dc.contributor.author Miró, Elisenda
• dc.contributor.author Iglesias-Torrens, Yaidelis
• dc.contributor.author Navarro, Ferran
• dc.contributor.author Campoy, Susana
• dc.contributor.author Alioto, Tyler
• dc.contributor.author Gómez-Garrido, Jèssica
• dc.contributor.author Madrid, Cristina
• dc.contributor.author Balsalobre, Carlos
• dc.date.accessioned 2022-05-24T10:33:13Z
• dc.date.available 2022-05-24T10:33:13Z
• dc.date.issued 2022
• dc.description.abstract Campylobacter jejuni is a foodborne pathogen causing bacterial gastroenteritis, with the highest incidence reported in Europe. The prevalence of antibiotic resistance in C. jejuni, as well as in many other bacterial pathogens, has increased over the last few years. In this report, we describe the presence of a plasmid in a multi-drug-resistant C. jejuni strain isolated from a gastroenteritis patient. Mating experiments demonstrated the transference of this genetic element (pCjH01) among C. jejuni by plasmid conjugation. The pCjH01 plasmid was sequenced and assembled, revealing high similarity (97% identity) with pTet, a described tetracycline resistance encoding plasmid. pCjH01 (47.7 kb) is a mosaic plasmid composed of a pTet backbone that has acquired two discrete DNA regions. Remarkably, one of the acquired sequences carried an undescribed variant of the aadE-sat4-aphA-3 gene cluster, providing resistance to at least kanamycin and gentamycin. Aside from the antibiotic resistance genes, the cluster also carries genes coding for putative regulators, such as a sigma factor of the RNA polymerase and an antisigma factor. Homology searches suggest that Campylobacter exchanges genetic material with distant G-positive bacterial genera.
• dc.description.sponsorship This research was funded by the Spanish Ministry of Economy and Competitiveness [grant AGL2013-45339R], the Spanish Ministry of Science, Innovation and Universities (MCIU), State Bureau of Investigation (AIE), the European Regional Development Fund (FEDER) [grant PGC2018-096958-B-I00], and the Catalonian government [grant 2017SGR499]. PG was the recipient of an ADR fellowship of the University of Barcelona
• dc.format.mimetype application/pdf
• dc.identifier.citation Guirado P, Miró E, Iglesias-Torrens Y, Navarro F, Campoy S, Alioto TS et al. A new variant of the aadE-sat4-aphA-3 gene cluster found in a conjugative plasmid from a MDR campylobacter jejuni isolate. Antibiotics (Basel). 2022 Mar 30;11(4):466. DOI:10.3390/antibiotics11040466
• dc.identifier.doi http://dx.doi.org/10.3390/antibiotics11040466
• dc.identifier.issn 2079-6382
• dc.identifier.uri http://hdl.handle.net/10230/53226
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2013-45339R
• dc.rights © 2022 by Pedro Guirado et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.other Infeccions per campilobàcter
• dc.subject.other Gastroenteritis
• dc.subject.other Bacteris
• dc.subject.other Antibiòtics
• dc.title A new variant of the aadE-sat4-aphA-3 gene cluster found in a conjugative plasmid from a MDR campylobacter jejuni isolate
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion