Remodeling of secretory compartments creates CUPS during nutrient starvation

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Cruz-Garcia, David
• dc.contributor.author Curwin, Amy
• dc.contributor.author Popoff, Jean-François
• dc.contributor.author Bruns, Caroline, 1984-
• dc.contributor.author Duran, Juan M.
• dc.contributor.author Malhotra, Vivek
• dc.date.accessioned 2023-12-11T06:48:07Z
• dc.date.available 2023-12-11T06:48:07Z
• dc.date.issued 2014
• dc.description Includes supplemental materials for the online appendix.
• dc.description.abstract Upon starvation, Grh1, a peripheral membrane protein located at endoplasmic reticulum (ER) exit sites and early Golgi in Saccharomyces cerevisiae under growth conditions, relocates to a compartment called compartment for unconventional protein secretion (CUPS). Here we report that CUPS lack Golgi enzymes, but contain the coat protein complex II (COPII) vesicle tethering protein Uso1 and the Golgi t-SNARE Sed5. Interestingly, CUPS biogenesis is independent of COPII- and COPI-mediated membrane transport. Pik1- and Sec7-mediated membrane export from the late Golgi is required for complete assembly of CUPS, and Vps34 is needed for their maintenance. CUPS formation is triggered by glucose, but not nitrogen starvation. Moreover, upon return to growth conditions, CUPS are absorbed into the ER, and not the vacuole. Altogether our findings indicate that CUPS are not specialized autophagosomes as suggested previously. We suggest that starvation triggers relocation of secretory and endosomal membranes, but not their enzymes, to generate CUPS to sort and secrete proteins that do not enter, or are not processed by enzymes of the ER–Golgi pathway of secretion.
• dc.description.sponsorship We acknowledge support from the Spanish Ministry of Economy and Competitiveness, Centro de Excelencia Severo Ochoa 2013-2017, SEV-2012-0208. V. Malhotra is an Institució Catalana de Recerca i Estudis Avançats (ICREA) professor at the Center for Genomic Regulation and the work in his laboratory is funded by grants from Plan Nacional (BFU2008-00414), Consolider (CSD2009-00016), and the European Research Council (268692). The project has received research funding from the European Union (EU). This paper reflects only the authors’ views. The EU is not liable for any use that may be made of the information contained therein.
• dc.format.mimetype application/pdf
• dc.identifier.citation Cruz-Garcia D, Curwin AJ, Popoff J, Bruns C, Duran JM, Malhotra V. Remodeling of secretory compartments creates CUPS during nutrient starvation. Journal of Cell Biology. 2014 Dec 22;207(6):695-703. DOI: 10.1083/jcb.201407119
• dc.identifier.doi http://dx.doi.org/10.1083/jcb.201407119
• dc.identifier.issn 0021-9525
• dc.identifier.uri http://hdl.handle.net/10230/58488
• dc.language.iso eng
• dc.publisher Rockefeller University Press
• dc.relation.ispartof Journal of Cell Biology. 2014 Dec 22;207(6):695-703
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/268692
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SEV-2012-0208
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2008-00414
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CSD2009-00016
• dc.rights © 2014 Cruz-Garcia et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/
• dc.subject.other Nutrició
• dc.subject.other Proteïnes
• dc.subject.other Enzims
• dc.title Remodeling of secretory compartments creates CUPS during nutrient starvation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion