Optimization of sTREM2 ELISA to understand the role of microglia in Alzheimer's disease
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- dc.contributor.author Miret Milian, Maria
- dc.date.accessioned 2023-07-26T10:29:52Z
- dc.date.available 2023-07-26T10:29:52Z
- dc.date.issued 2023
- dc.description Treball de fi de grau en Biologia Humanaca
- dc.description Directors: Marc Suárez-Calvet, Federica Anastasi
- dc.description Tutor acadèmic: Francisco José Muñoz López (Universitat Pompeu Fabra)
- dc.description.abstract Triggering receptor expressed on myeloid cells 2 (TREM2), a microglial receptor, plays a crucial role in the innate immune response in Alzheimer's disease (AD), aiding in the clearance of amyloid beta plaques and preventing Tau propagation. As a result of cleavage, soluble TREM2 (sTREM2) can be detected in human cerebrospinal fluid (CSF) and plasma across the AD continuum, serving as a biomarker of microglial activity. This study aimed to optimize a Meso Scale Discovery (MSD) enzyme-linked immunosorbent assay (ELISA) for the quantification of sTREM2 in biofluids. Validation parameters considered for optimization included signal-to-noise (S/N) ratio, background noise and coefficient of variation (CV%). Optimal capture and detection antibodies concentrations were determined to be 15.63 ng/ml and 0.125 μg/ml, respectively, resulting in improved assay performance. Despite higher background noise, storing MSD reagents at 4ºC and the use of MSD Small Spot coated-streptavidin plates improved the S/N ratio. Finally, a concentration of 0.0625 μg/ml of secondary antibody rendered a lower background and higher S/N ratio. These optimizations significantly increased the sensitivity and accuracy of the immunoassay, making it a promising tool for the early detection of pathological alterations linked to AD.ca
- dc.format.mimetype application/pdf
- dc.identifier.uri http://hdl.handle.net/10230/57678
- dc.language.iso engca
- dc.rights Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional (CC BY-NC-ND 4.0)
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ca
- dc.subject.other Alzheimer, Malaltia d'
- dc.subject.other Micròglia
- dc.title Optimization of sTREM2 ELISA to understand the role of microglia in Alzheimer's diseaseca
- dc.type info:eu-repo/semantics/bachelorThesisca