Sampling time-dependent artifacts in single-cell genomics studies
Sampling time-dependent artifacts in single-cell genomics studies
Citació
- Massoni-Badosa R, Iacono G, Moutinho C, Kulis M, Palau N, Marchese D et al. Sampling time-dependent artifacts in single-cell genomics studies. Genome Biol. 2020 May 11; 21(1): 112. DOI: 10.1186/s13059-020-02032-0
Enllaç permanent
Descripció
Resum
Robust protocols and automation now enable large-scale single-cell RNA and ATAC sequencing experiments and their application on biobank and clinical cohorts. However, technical biases introduced during sample acquisition can hinder solid, reproducible results, and a systematic benchmarking is required before entering large-scale data production. Here, we report the existence and extent of gene expression and chromatin accessibility artifacts introduced during sampling and identify experimental and computational solutions for their prevention.