Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide
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- dc.contributor.author Blanco, Esther
- dc.contributor.author Guerra, Beatriz
- dc.contributor.author Torre, Beatriz G. de la
- dc.contributor.author Defaus, Sira
- dc.contributor.author Dekker, Aldo
- dc.contributor.author Andreu Martínez, David
- dc.contributor.author Sobrino, Francisco
- dc.date.accessioned 2025-11-04T15:41:24Z
- dc.date.available 2025-11-04T15:41:24Z
- dc.date.issued 2016
- dc.date.updated 2025-11-04T15:41:24Z
- dc.description.abstract Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136-154)] linked through thioether bonds to a T-cell epitope [3A(21-35)] of FMDV [B4T(thi)] elicits potent B- and T-cell specific responses and confers solid protection in pigs to type C FMDV challenge. Herein we show that downsized versions of this peptide bearing two copies of a B-cell epitope from a type O isolate and using thioether [B2T(thi)] or maleimide [B2T(mal)] conjugation chemistries for their synthesis elicited in swine similar or higher B and T-cell specific responses than tetravalent B4T(thi). Moreover, while partial protection was observed in animals immunized with B4T(thi) (60%) and B2T(thi) (80%), B2T(mal) conferred full (100%) protection against FMDV challenge, associated to high levels of circulating IgG2 and mucosal IgGA, and entirely prevented virus shedding. Interestingly, B2T(mal) is also the most advantageous option in terms of synthetic practicality. Taken together, the results reported here point out to B2T(mal) as a highly valuable, cost-effective FMDV candidate vaccine.
- dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness (grants SAF2011-24899, and AGL2014-52395-C2 to FS and DA; AGL2013-48923-C2 to EB), the European Community's Seventh Framework Programme (FP7, 2007-2013), Research Infrastructures action (NADIR), under the grant agreement No. FP7-228394 (to FS, DA, EB and AD), Comunidad de Madrid (S2013/ABI-2906-PLATESA to FS and EB) and Generalitat de Catalunya (2009SGR492 to DA). Work at Centro de Biología Molecular "Severo Ochoa" was supported by Fundación Ramón Areces.
- dc.format.mimetype application/pdf
- dc.identifier.citation Blanco E, Guerra B, Garcia de la Torre B, Defaus S, Dekker A, Andreu D, Sobrino F. Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide. Antiviral Res. 2016 May:129:74-80. DOI: 10.1016/j.antiviral.2016.03.005
- dc.identifier.doi http://dx.doi.org/10.1016/j.antiviral.2016.03.005
- dc.identifier.issn 0166-3542
- dc.identifier.uri http://hdl.handle.net/10230/71764
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Antiviral Research. 2016;129:74-80
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2011-24899
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2014-52395-C2
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2013-48923-C2
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/228394
- dc.rights © Elsevier http://dx.doi.org/10.1016/j.antiviral.2016.03.005
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Foot-and-mouth disease virus
- dc.subject.keyword Peptide dendrimers
- dc.subject.keyword Vaccines
- dc.subject.keyword Protection
- dc.title Full protection of swine against foot-and-mouth disease by a bivalent B-cell epitope dendrimer peptide
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion