Prenatal exposure to organophosphate pesticides and brain morphology and white matter microstructure in preadolescents

Dries, Michiel A. van den; Lamballais, Sander; El Marroun, Hanan; Pronk, Anjoeka; Spaan, Suzanne; Ferguson, Kelly K.; Longnecker, Matthew P.; Tiemeier, Henning; Guxens Junyent, Mònica

doi:http://dx.doi.org/10.1016/j.envres.2020.110047

Prenatal exposure to organophosphate pesticides and brain morphology and white matter microstructure in preadolescents

Citation

Van den Dries MA, Lamballais S, El Marroun H, Pronk A, Spaan S, Ferguson KK et al. Prenatal exposure to organophosphate pesticides and brain morphology and white matter microstructure in preadolescents. Environ Res. 2020 Dec;191:110047. DOI: 10.1016/j.envres.2020.110047

Abstract

Background: Prenatal exposure to organophosphate (OP) pesticides associate with impaired neurodevelopment in humans and animal models. However, much uncertainty exists about the brain structural alterations underlying these associations. The objective of this study was to determine whether maternal OP pesticide metabolite concentrations in urine repeatedly measured during gestation are associated with brain morphology and white matter microstructure in 518 preadolescents aged 9-12 years. Method: Data came from 518 mother-child pairs participating in the Generation R Study, a population-based birth cohort from Rotterdam, the Netherlands. Maternal urine concentrations were determined for 6 dialkylphosphates (DAPs) including 3 dimethyl (DM) and 3 diethyl (DE) alkyl phosphate metabolites, collected at early, mid, and late pregnancy. At child's age 9-12 years, magnetic resonance imaging was performed to obtain T1-weighted images for brain volumes and surface-based cortical thickness and cortical surface area, and diffusion tensor imaging was used to measure white matter microstructure through fractional anisotropy (FA) and mean diffusivity (MD). Linear regression models were fit for the averaged prenatal exposure across pregnancy. Results: DM and DE metabolite concentrations were not associated with brain volumes, cortical thickness, and cortical surface area. However, a 10-fold increase in averaged DM metabolite concentrations across pregnancy was associated with lower FA (B = -1.00, 95%CI = -1.80, -0.20) and higher MD (B = 0.13, 95%CI = 0.04, 0.21). Similar associations were observed for DE concentrations. Conclusions: This study provides the first evidence that OP pesticides may alter normal white matter microstructure in children, which could have consequences for normal neurodevelopment. No associations were observed with structural brain morphology, including brain volumes, cortical thickness, and cortical surface area.