Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories

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• dc.contributor.author Sánchez Muñoz, Raúl, 1990-
• dc.contributor.author Moyano Claramunt, Elisabet
• dc.contributor.author Khojasteh, Abbas
• dc.contributor.author Bonfill Baldrich, Ma. Mercedes
• dc.contributor.author Cusidó Vidal, Rosa M.
• dc.contributor.author Palazón Barandela, Javier
• dc.date.accessioned 2025-01-29T13:56:32Z
• dc.date.available 2025-01-29T13:56:32Z
• dc.date.issued 2019
• dc.description.abstract Plant cell biofactories offer great advantages for the production of plant compounds of interest, although certain limitations still need to be overcome before their maximum potential is reached. One obstacle is the gradual loss of secondary metabolite production during in vitro culture maintenance, which is an important impediment in the development of large-scale production systems. The relationship between in vitro maintenance and epigenetic changes has been demonstrated in several plant species; in particular, methylation levels have been found to increase in in vitro cultures over time. Higher DNA methylation levels have been correlated with a low yield of secondary metabolites in in vitro plant cell cultures. The longer the period of subculturing, the more methylated cytosines were found throughout the genome, and secondary metabolism decreased significantly. This review summarizes different studies on epigenetic changes during the maintenance of in vitro cell cultures and the insights they provide on the mechanisms involved. It concludes by looking at the perspectives for new approaches designed to avoid declines in metabolite production.
• dc.format.mimetype application/pdf
• dc.identifier.citation Sanchez-Muñoz R, Moyano E, Khojasteh A, Bonfill M, Cusido RM, Palazon J. Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories. Eng Life Sci. 2019 May 17;19(12):872-9. DOI: 10.1002/elsc.201900024
• dc.identifier.doi http://dx.doi.org/10.1002/elsc.201900024
• dc.identifier.issn 1618-0240
• dc.identifier.uri http://hdl.handle.net/10230/69358
• dc.language.iso eng
• dc.publisher Wiley
• dc.relation.ispartof Eng Life Sci. 2019 May 17;19(12):872-9
• dc.rights This is the peer reviewed version of the following article: Sanchez-Muñoz R, Moyano E, Khojasteh A, Bonfill M, Cusido RM, Palazon J. Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories. Eng Life Sci. 2019 May 17;19(12):872-9. DOI: 10.1002/elsc.201900024, which has been published in final form at http://dx.doi.org/10.1002/elsc.201900024. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Cell cultures
• dc.subject.keyword Epigenetic
• dc.subject.keyword Methylation
• dc.subject.keyword Natural products
• dc.subject.keyword Plant biofactories
• dc.title Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion