Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept

Gomez‑Gonzalez, Emilio; Barriga‑Rivera, Alejandro; Fernandez‑Muñoz, Beatriz; Navas‑Garcia, Jose Manuel; Fernandez‑Lizaranzu, Isabel; Muñoz‑Gonzalez, Francisco Javier; Parrilla‑Giraldez, Ruben; Requena‑Lancharro, Desiree; Gil‑Gamboa, Pedro; Rosell‑Valle, Cristina; Gomez‑Gonzalez, Carmen; Mayorga‑Buiza, Maria Jose; Martin‑Lopez, Maria; Muñoz, Olga; Gomez‑Martin, Juan Carlos; Relimpio‑Lopez, Maria Isabel; Aceituno‑Castro, Jesus; Perales‑Esteve, Manuel A.; Puppo‑Moreno, Antonio; Garcia‑Cozar, Francisco Jose; Olvera‑Collantes, Lucia; Gomez‑Diaz, Raquel; Santos‑Trigo, Silvia de los; Huguet‑Carrasco, Monserrat; Rey, Manuel; Gómez Gutiérrez, Emilia, 1975-; Sanchez‑Pernaute, Rosario; Padillo‑Ruiz, Javier; Marquez‑Rivas, Javier

Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept

Mostra el registre complet Registre parcial de l'ítem

dc.contributor.author Gomez‑Gonzalez, Emilio
dc.contributor.author Barriga‑Rivera, Alejandro
dc.contributor.author Fernandez‑Muñoz, Beatriz
dc.contributor.author Navas‑Garcia, Jose Manuel
dc.contributor.author Fernandez‑Lizaranzu, Isabel
dc.contributor.author Muñoz‑Gonzalez, Francisco Javier
dc.contributor.author Parrilla‑Giraldez, Ruben
dc.contributor.author Requena‑Lancharro, Desiree
dc.contributor.author Gil‑Gamboa, Pedro
dc.contributor.author Rosell‑Valle, Cristina
dc.contributor.author Gomez‑Gonzalez, Carmen
dc.contributor.author Mayorga‑Buiza, Maria Jose
dc.contributor.author Martin‑Lopez, Maria
dc.contributor.author Muñoz, Olga
dc.contributor.author Gomez‑Martin, Juan Carlos
dc.contributor.author Relimpio‑Lopez, Maria Isabel
dc.contributor.author Aceituno‑Castro, Jesus
dc.contributor.author Perales‑Esteve, Manuel A.
dc.contributor.author Puppo‑Moreno, Antonio
dc.contributor.author Garcia‑Cozar, Francisco Jose
dc.contributor.author Olvera‑Collantes, Lucia
dc.contributor.author Gomez‑Diaz, Raquel
dc.contributor.author Santos‑Trigo, Silvia de los
dc.contributor.author Huguet‑Carrasco, Monserrat
dc.contributor.author Rey, Manuel
dc.contributor.author Gómez Gutiérrez, Emilia, 1975-
dc.contributor.author Sanchez‑Pernaute, Rosario
dc.contributor.author Padillo‑Ruiz, Javier
dc.contributor.author Marquez‑Rivas, Javier
dc.date.accessioned 2023-02-22T07:25:34Z
dc.date.available 2023-02-22T07:25:34Z
dc.date.issued 2022
dc.description.abstract Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.
dc.description.sponsorship This research was funded by Grants Number COV20-00080 and COV20-00173 of the 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease of the Institute of Health ‘Carlos III’, Spanish Ministry of Science and Innovation, and by Grant Number EQC2019-006240-P funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. ABR was supported by Grant Number RTI2018-094465-J-I00 funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. This work has been supported by the European Commission through the Joint Research Center (JRC) HUMAINT project.
dc.format.mimetype application/pdf
dc.identifier.citation Gomez‑Gonzalez E, Barriga‑Rivera A, Fernandez‑Muñoz B, Navas‑Garcia JM, Fernandez‑Lizaranzu I, Munoz‑Gonzalez FJ, Parrilla‑Giraldez R, Requena‑Lancharro D, Gil‑Gamboa P, Rosell-Valle C, Gomez‑Gonzalez C, Mayorga‑Buiza MJ, Martin‑Lopez M, Muñoz O, Gomez‑Martin JC, Relimpio‑Lopez MI, Aceituno‑Castro J, Perales‑Esteve MA, Puppo‑Moreno A, Garcia‑Cozar FJ, Olvera‑Collantes L, Gomez‑Diaz R, de los Santos‑Trigo S, Huguet‑Carrasco M, Rey M, Gomez E, Sanchez‑Pernaute R, Padillo‑Ruiz J, Marquez‑Rivas J. Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept. Sci Rep. 2022;12:2356. DOI: 10.1038/s41598-022-06393-3
dc.identifier.doi http://dx.doi.org/10.1038/s41598-022-06393-3
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10230/55846
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartof Scientific Reports. 2022;12:2356.
dc.relation.isreferencedby https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-022-06393-3/MediaObjects/41598_2022_6393_MOESM1_ESM.docx
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018-094465-J-I00
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/EQC2019-006240-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other COVID-19 (Malaltia)
dc.subject.other Imatges--Processament
dc.subject.other Intel·ligència artificial
dc.title Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Departament de Tecnologies de la Informació i les Comunicacions)