Regulation of lipid droplets by metabolically controlled Ldo isoforms

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  • dc.contributor.author Teixeira, Vítor
  • dc.contributor.author Martínez-Montañés, Fernando
  • dc.contributor.author Grippa, Alexandra, 1980-
  • dc.contributor.author Buxó, Laura
  • dc.contributor.author Idrissi, Fatima-Zahra
  • dc.contributor.author Ejsing, Christer S.
  • dc.contributor.author Carvalho, Pedro C.
  • dc.date.accessioned 2019-11-21T12:50:39Z
  • dc.date.available 2019-11-21T12:50:39Z
  • dc.date.issued 2018
  • dc.description.abstract Storage and consumption of neutral lipids in lipid droplets (LDs) are essential for energy homeostasis and tightly coupled to cellular metabolism. However, how metabolic cues are integrated in the life cycle of LDs is unclear. In this study, we characterize the function of Ldo16 and Ldo45, two splicing isoforms of the same protein in budding yeast. We show that Ldo proteins interact with the seipin complex, which regulates contacts between LDs and the endoplasmic reticulum (ER). Moreover, we show that the levels of Ldo16 and Ldo45 depend on the growth stage of cells and that deregulation of their relative abundance alters LD morphology, protein localization, and triglyceride content. Finally, we show that absence of Ldo proteins results in defects in LD morphology and consumption by lipophagy. Our findings support a model in which Ldo proteins modulate the activity of the seipin complex, thereby affecting LD properties. Moreover, we identify ER-LD contacts as regulatory targets coupling energy storage to cellular metabolism.
  • dc.description.sponsorship L. Johnsen was supported by a La Caixa Foundation graduate fellowship. P. Carvalho is supported by a European Research Council starting grant (FP7/2007–2013 European Research Council grant agreement 309477 DROPFAT) and the European Molecular Biology Organization Young Investors Program.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Teixeira V, Johnsen L, Martínez-Montañés F, Grippa A, Buxó L, Idrissi FZ, Ejsing CS, Carvalho P. Regulation of lipid droplets by metabolically controlled Ldo isoforms. J Cell Biol. 2018; 217(1):127-138. DOI 10.1083/jcb.201704115
  • dc.identifier.doi http://dx.doi.org/10.1083/jcb.201704115
  • dc.identifier.issn 0021-9525
  • dc.identifier.uri http://hdl.handle.net/10230/42920
  • dc.language.iso eng
  • dc.publisher Rockefeller University Press
  • dc.relation.ispartof J Cell Biol. 2018; 217(1):127-138
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/309477
  • dc.rights © 2018 Teixeira et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri https://creativecommons.org/licenses/by/4.0/
  • dc.title Regulation of lipid droplets by metabolically controlled Ldo isoforms
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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