FUS phase separation is modulated by a molecular chaperone and methylation of arginine cation-π interactions

Qamar, Seema; Tartaglia, Gian Gaetano; St George-Hyslop, Peter

doi:http://dx.doi.org/10.1016/j.cell.2018.03.056

FUS phase separation is modulated by a molecular chaperone and methylation of arginine cation-π interactions

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Qamar S, Wang G, Randle SJ, Ruggeri FS, Varela JA, Lin JQ et al. FUS phase separation is modulated by a molecular chaperone and methylation of arginine cation-π interactions. Cell. 2018;173(3):720-34. DOI: 10.1016/j.cell.2018.03.056

Abstract

Reversible phase separation underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles and is, in part, driven by the intrinsically disordered low-complexity (LC) domain of FUS. Here, we report that cooperative cation-π interactions between tyrosines in the LC domain and arginines in structured C-terminal domains also contribute to phase separation. These interactions are modulated by post-translational arginine methylation, wherein arginine hypomethylation strongly promotes phase separation and gelation. Indeed, significant hypomethylation, which occurs in FUS-associated frontotemporal lobar degeneration (FTLD), induces FUS condensation into stable intermolecular β-sheet-rich hydrogels that disrupt RNP granule function and impair new protein synthesis in neuron terminals. We show that transportin acts as a physiological molecular chaperone of FUS in neuron terminals, reducing phase separation and gelation of methylated and hypomethylated FUS and rescuing protein synthesis. These results demonstrate how FUS condensation is physiologically regulated and how perturbations in these mechanisms can lead to disease.