Long noncoding RNAs with enhancer-like function in human cells
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- dc.contributor.author Orom, Ulf Anderssonca
- dc.contributor.author Derrien, Thomasca
- dc.contributor.author Beringer, Malteca
- dc.contributor.author Gumireddy, Kiranmaica
- dc.contributor.author Gardini, Alessandroca
- dc.contributor.author Bussotti, Giovanni, 1983-ca
- dc.contributor.author Lai, Fanca
- dc.contributor.author Zytnicki, Matthiasca
- dc.contributor.author Notredame, Cedricca
- dc.contributor.author Huang, Qihongca
- dc.contributor.author Guigó Serra, Rodericca
- dc.contributor.author Shiekhattar, Raminca
- dc.date.accessioned 2016-01-07T15:42:32Z
- dc.date.available 2016-01-07T15:42:32Z
- dc.date.issued 2010
- dc.description.abstract While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in human cell lines. Depletion of a number of ncRNAs led to decreased expression of their neighboring protein-coding genes, including the master regulator of hematopoiesis, SCL (also called TAL1), Snai1 and Snai2. Using heterologous transcription assays we demonstrated a requirement for the ncRNAs in activation of gene expression. These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation.ca
- dc.format.mimetype application/pdfca
- dc.identifier.citation Orom UA, Derrien T, Beringer M, Gumireddy K, Gardini A, Bussotti G et al. Long noncoding RNAs with enhancer-like function in human cells. Cell. 2010; 143(1): 46-58. DOI 10.1016/j.cell.2010.09.001ca
- dc.identifier.doi http://dx.doi.org/10.1016/j.cell.2010.09.001
- dc.identifier.issn 0092-8674
- dc.identifier.uri http://hdl.handle.net/10230/25539
- dc.language.iso engca
- dc.publisher Elsevierca
- dc.relation.ispartof Cell. 2010; 143(1): 46-58
- dc.rights © Elsevier. This is the published version of an article http://dx.doi.org/10.1016/j.cell.2010.09.001 that appeared in the journal Cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.other RNA missatgerca
- dc.subject.other Factors de transcripcióca
- dc.title Long noncoding RNAs with enhancer-like function in human cellsca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca