The alternative splicing factor Nova2 regulates vascular development and lumen formation
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- dc.contributor.author Giampietro, Costanzaca
- dc.contributor.author Wyatt, Christopher Douglas Robert, 1988-ca
- dc.contributor.author Irimia Martínez, Manuelca
- dc.contributor.author Ghigna, Claudiaca
- dc.date.accessioned 2015-11-17T13:22:07Z
- dc.date.available 2015-11-17T13:22:07Z
- dc.date.issued 2015
- dc.description.abstract Vascular lumen formation is a fundamental step during angiogenesis; yet, the molecular mechanisms underlying this process are poorly understood. Recent studies have shown that neural and vascular systems share common anatomical, functional and molecular similarities. Here we show that the organization of endothelial lumen is controlled at the post-transcriptional level by the alternative splicing (AS) regulator Nova2, which was previously considered to be neural cell-specific. Nova2 is expressed during angiogenesis and its depletion disrupts vascular lumen formation in vivo. Similarly, Nova2 depletion in cultured endothelial cells (ECs) impairs the apical distribution and the downstream signalling of the Par polarity complex, resulting in altered EC polarity, a process required for vascular lumen formation. These defects are linked to AS changes of Nova2 target exons affecting the Par complex and its regulators. Collectively, our results reveal that Nova2 functions as an AS regulator in angiogenesis and is a novel member of the 'angioneurins' family.en
- dc.format.mimetype application/pdfca
- dc.identifier.citation Giampietro C, Deflorian G, Gallo S, Di Matteo A, Pradella D, Bonomi S et al. The alternative splicing factor Nova2 regulates vascular development and lumen formation. Nature communications. 2015;6:8479. DOI: 10.1038/ncomms9479ca
- dc.identifier.doi http://dx.doi.org/10.1038/ncomms9479
- dc.identifier.issn 2041-1723
- dc.identifier.uri http://hdl.handle.net/10230/25112
- dc.language.iso engca
- dc.publisher Nature Publishing Groupca
- dc.relation.ispartof Nature communications. 2015;6:8479
- dc.rights © 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
- dc.subject.other Angiogènesi
- dc.subject.other Neurones -- Fisiologia
- dc.title The alternative splicing factor Nova2 regulates vascular development and lumen formationen
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca