NEAT1 long isoform is highly expressed in chronic lymphocytic leukemia irrespectively of cytogenetic groups or clinical outcome

Ronchetti, Domenica; Favasuli, Vanessa; Monti, Paola; Cutrona, Giovanna; Fabris, Sonia; Silvestris, Ilaria; Agnelli, Luca; Colombo, Monica; Menichini, Paola; Matis, Serena; Gentile, Massimo; Nurtdinov, Ramil; Guigó Serra, Roderic; Baldini, Luca; Fronza, Gilberto; Ferrarini, Manlio; Morabito, Fortunato; Neri, Antonino; Taiana, Elisa

NEAT1 long isoform is highly expressed in chronic lymphocytic leukemia irrespectively of cytogenetic groups or clinical outcome

Mostra el registre complet Registre parcial de l'ítem

dc.contributor.author Ronchetti, Domenica
dc.contributor.author Favasuli, Vanessa
dc.contributor.author Monti, Paola
dc.contributor.author Cutrona, Giovanna
dc.contributor.author Fabris, Sonia
dc.contributor.author Silvestris, Ilaria
dc.contributor.author Agnelli, Luca
dc.contributor.author Colombo, Monica
dc.contributor.author Menichini, Paola
dc.contributor.author Matis, Serena
dc.contributor.author Gentile, Massimo
dc.contributor.author Nurtdinov, Ramil
dc.contributor.author Guigó Serra, Roderic
dc.contributor.author Baldini, Luca
dc.contributor.author Fronza, Gilberto
dc.contributor.author Ferrarini, Manlio
dc.contributor.author Morabito, Fortunato
dc.contributor.author Neri, Antonino
dc.contributor.author Taiana, Elisa
dc.date.accessioned 2020-05-12T07:22:34Z
dc.date.available 2020-05-12T07:22:34Z
dc.date.issued 2020
dc.description.abstract The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in chronic lymphocytic leukemia (CLL) are still open questions. Herein, we investigated the significance of the lncRNA NEAT1 in CLL. We examined NEAT1 expression in 310 newly diagnosed Binet A patients, in normal CD19+ B-cells, and other types of B-cell malignancies. Although global NEAT1 expression level was not statistically different in CLL cells compared to normal B cells, the median ratio of NEAT1_2 long isoform and global NEAT1 expression in CLL samples was significantly higher than in other groups. NEAT1_2 was more expressed in patients carrying mutated IGHV genes. Concerning cytogenetic aberrations, NEAT1_2 expression in CLL with trisomy 12 was lower with respect to patients without alterations. Although global NEAT1 expression appeared not to be associated with clinical outcome, patients with the lowest NEAT1_2 expression displayed the shortest time to first treatment; however, a multivariate regression analysis showed that the NEAT1_2 risk model was not independent from other known prognostic factors, particularly the IGHV mutational status. Overall, our data prompt future studies to investigate whether the increased amount of the long NEAT1_2 isoform detected in CLL cells may have a specific role in the pathology of the disease.
dc.description.sponsorship This work was financially supported by grants to Antonino Neri [from Associazione Italiana Ricerca sul Cancro (AIRC) (IG16722, IG10136, and the “Special Program Molecular Clinical Oncology-5 per mille” #9980, 2010/15)]; to Giovanna Cutrona and Gilberto Fronza [from the Italian Ministry of Health 5 × 1000 funds 2014, 2015, 2016, and from the Compagnia S. Paolo Turin Italy (project 2017.0526)]; to Manlio Ferrarini (the “Special Program Molecular Clinical Oncology-5 per mille” #9980 and AIRC I.G. n.14326); to Fortunato Morabito (the “Special Program Molecular Clinical Oncology-5 per mille” #9980 and AIRC and Fondazione CaRiCal co-financed Multi-Unit Regional Grant 2014 n.16695); Elisa Taiana was supported by a fellowship (#19370) from the Fondazione Italiana Ricerca sul cancro (FIRC)
dc.format.mimetype application/pdf
dc.identifier.citation Ronchetti D, Favasuli V, Monti P, Cutrona G, Fabris S, Silvestris I et al. NEAT1 long isoform is highly expressed in chronic lymphocytic leukemia irrespectively of cytogenetic groups or clinical outcome. Noncoding RNA. 2020 Mar 13; 6(1). pii: E11. DOI: 10.3390/ncrna6010011
dc.identifier.doi http://dx.doi.org/10.3390/ncrna6010011
dc.identifier.issn 2311-553X
dc.identifier.uri http://hdl.handle.net/10230/44496
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof Non-coding RNA. 2020 Mar 13;6(1).pii: E11
dc.rights © 2020 by Domenica Ronchetti et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Leucèmia limfoide
dc.subject.other RNA
dc.title NEAT1 long isoform is highly expressed in chronic lymphocytic leukemia irrespectively of cytogenetic groups or clinical outcome
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Center for Genomic Regulation (CRG))