Yeast Cip1 is activated by environmental stress to inhibit Cdk1–G1 cyclins via Mcm1 and Msn2/4

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• dc.contributor.author Chang, Ya-Lanca
• dc.contributor.author Tseng, Shun-Fuca
• dc.contributor.author Huang, Yu-Chingca
• dc.contributor.author Shen, Zih-Jieca
• dc.contributor.author Hsu, Pang-Hungca
• dc.contributor.author Hsieh, Meng-Hsunca
• dc.contributor.author Yang, Chia-Weica
• dc.contributor.author Tognetti, Silviaca
• dc.contributor.author Canal de Torres, Berta, 1988-ca
• dc.contributor.author Subirana, Laiaca
• dc.contributor.author Wang, Chien-Weica
• dc.contributor.author Chen, Hsiao-Tanca
• dc.contributor.author Lin, Chi-Yingca
• dc.contributor.author Posas Garriga, Francescca
• dc.contributor.author Teng, Shu-Chunca
• dc.date.accessioned 2018-01-19T11:19:12Z
• dc.date.available 2018-01-19T11:19:12Z
• dc.date.issued 2017
• dc.description.abstract Upon environmental changes, proliferating cells delay cell cycle to prevent further damage accumulation. Yeast Cip1 is a Cdk1 and Cln2-associated protein. However, the function and regulation of Cip1 are still poorly understood. Here we report that Cip1 expression is co-regulated by the cell-cycle-mediated factor Mcm1 and the stress-mediated factors Msn2/4. Overexpression of Cip1 arrests cell cycle through inhibition of Cdk1-G1 cyclin complexes at G1 stage and the stress-activated protein kinase-dependent Cip1 T65, T69, and T73 phosphorylation may strengthen the Cip1and Cdk1-G1 cyclin interaction. Cip1 accumulation mainly targets Cdk1-Cln3 complex to prevent Whi5 phosphorylation and inhibit early G1 progression. Under osmotic stress, Cip1 expression triggers transient G1 delay which plays a functionally redundant role with another hyperosmolar activated CKI, Sic1. These findings indicate that Cip1 functions similarly to mammalian p21 as a stress-induced CDK inhibitor to decelerate cell cycle through G1 cyclins to cope with environmental stresses.A G1 cell cycle regulatory kinase Cip1 has been identified in budding yeast but how this is regulated is unclear. Here the authors identify cell cycle (Mcm1) and stress-mediated (Msn 2/4) transcription factors as regulating Cip1, causing stress induced CDK inhibition and delay in cell cycle progression.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Chang YL, Tseng SF, Huang YC, Shen ZJ, Hsu PH, Hsieh MH et al. Yeast Cip1 is activated by environmental stress to inhibit Cdk1–G1 cyclins via Mcm1 and Msn2/4. Nat Commun. 2017 Jul 4;8(1):56. DOI: 10.1038/s41467-017-00080-y
• dc.identifier.doi http://dx.doi.org/10.1038/s41467-017-00080-y
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/33695
• dc.language.iso eng
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof Nature Communications. 2017 Jul 4;8(1):56
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-64437-P
• dc.rights © Nature Publishing Group. http://dx.doi.org/10.1038/s41467-017-00080-y. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Checkpoints
• dc.subject.keyword Phosphorylation
• dc.subject.keyword Saccharomyces cerevisiae
• dc.title Yeast Cip1 is activated by environmental stress to inhibit Cdk1–G1 cyclins via Mcm1 and Msn2/4ca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion