Soluble guanylate cyclase stimulation reduces oxidative stress in experimental Chronic Obstructive Pulmonary Disease
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- dc.contributor.author Paul, Tanja
- dc.contributor.author Salazar Degracia, Anna
- dc.contributor.author Peinado, Victor I.
- dc.contributor.author Tura-Ceide, Olga
- dc.contributor.author Blanco, Isabel
- dc.contributor.author Barreiro Portela, Esther
- dc.contributor.author Barberà, Joan Albert
- dc.date.accessioned 2019-01-15T08:04:42Z
- dc.date.available 2019-01-15T08:04:42Z
- dc.date.issued 2018
- dc.description.abstract OBJECTIVE: Soluble guanylate cyclase (sGC) is a key enzyme of the nitric oxide-cyclic guanosine 3',5'-monophosphate (NO-cGMP) signaling pathway, and its pharmacological stimulation has been shown to prevent the development of emphysema and pulmonary vascular remodeling in animal models of chronic obstructive pulmonary disease (COPD). The aim of this study was to evaluate the effects of sGC stimulation on oxidative stress in the plasma of guinea pigs chronically exposed to cigarette smoke (CS). METHODS AND RESULTS: Guinea pigs were exposed to CS or sham for three months, and received either the sGC stimulator BAY 41-2272 or vehicle. Body weight was measured weekly; and markers of oxidative stress in plasma, and airspace size and inflammatory cell infiltrate in lung tissue were analyzed at the end of the study. Compared to sham-exposed guinea pigs, CS-exposed animals gained less body weight and showed higher plasma levels of nitrated tyrosine residues (3-NT), 4-hydroxynonenal (4-HNE), and 8-hydroxydeoxyguanosine (8-OHdG). Treatment with the sGC stimulator led to a body weight gain in the CS-exposed guinea pigs similar to non-exposed and attenuated the increase in 3-NT and 4-HNE. Plasma levels of 3-NT correlated with the severity of inflammatory cell infiltrate in the lung. CONCLUSION: Stimulation of sGC prevents oxidative stress induced by CS exposure and is associated with an attenuated inflammatory response in the lung.
- dc.format.mimetype application/pdf
- dc.identifier.citation Paul T, Salazar-Degracia A, Peinado VI, Tura-Ceide O, Blanco I, Barreiro E. et al. Soluble guanylate cyclase stimulation reduces oxidative stress in experimental Chronic Obstructive Pulmonary Disease. PLoS One. 2018 Jan 5;13(1):e0190628. DOI: 10.1371/journal.pone.0190628
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0190628
- dc.identifier.issn 1932-6203
- dc.identifier.uri http://hdl.handle.net/10230/36265
- dc.language.iso eng
- dc.publisher Public Library of Science (PLoS)
- dc.relation.ispartof PLoS One. 2018 Jan 5;13(1):e0190628
- dc.rights Copyright © 2018 Paul et al. This is an open access article distributed under the https://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Pulmons -- Malalties obstructives
- dc.subject.other Estrés oxidatiu
- dc.title Soluble guanylate cyclase stimulation reduces oxidative stress in experimental Chronic Obstructive Pulmonary Disease
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion