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Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs

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dc.contributor.author Bohórquez, José Alejandro
dc.contributor.author Defaus, Sira
dc.contributor.author Rosell, Rosa
dc.contributor.author Pérez-Simó, Marta
dc.contributor.author Alberch, Mònica
dc.contributor.author Gladue, Douglas P.
dc.contributor.author Borca, Manuel V.
dc.contributor.author Andreu Martínez, David
dc.contributor.author Ganges, Lliliane
dc.date.accessioned 2021-11-12T07:22:08Z
dc.date.available 2021-11-12T07:22:08Z
dc.date.issued 2021
dc.identifier.citation Bohórquez JA, Defaus S, Rosell R, Pérez-Simó M, Alberch M, Gladue DP, Borca MV, Andreu D, Ganges L. Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs. Viruses. 2021;13(10):1980. DOI: 10.3390/v13101980
dc.identifier.issn 1999-4915
dc.identifier.uri http://hdl.handle.net/10230/48960
dc.description.abstract Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof Viruses. 2021;13(10):1980
dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3390/v13101980
dc.subject.keyword CSFV live-attenuated vaccine
dc.subject.keyword FlagT4G CSFV vaccine candidate
dc.subject.keyword Serological DIVA test
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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