Browsing by Author "Beato, Miguel"

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  • 90 YEARS OF PROGESTERONE: Molecular mechanisms of progesterone receptor action on the breast cancer genome
    Beato, Miguel; Wright, Roni H.G.; Le Dily, François (BioScientifica, 2020)
    Gene regulation by steroid hormones has been at the forefront in elucidating the intricacies of transcriptional regulation in eukaryotes ever since the discovery by Karlson and Clever that the insect steroid hormone ecdysone ...
  • A 3D culture system to study gene regulation in breast cancer cells
    Ramírez Cuéllar, Angélica Julieta (2022-05-16)
    In this Thesis we report the successful growth of hormone responsive T47D cells as 3D spheroids in Matrigel mimicking the ECM. A detailed characterization by using a genome-wide approach of the main physical and biochemical ...
  • A progesterone derivative linked to a stable phospholipid activates breast cancer cell response without leaving the cell membrane
    Font Mateu, Jofre, 1977-; Sanllehí, Pol; Sot, Jesús; Abad, Beatriz; Mateos, Nicolas; Torreno-Pina, Juan A.; Ferrari, Roberto; Wright, Roni H.G.; Garcia-Parajo, Maria F.; Joglar Tamargo, Jesús; Goñi, Félix M.; Beato, Miguel (Springer, 2024)
    In hormone-responsive breast cancer cells, progesterone (P4) has been shown to act via its nuclear receptor (nPR), a ligand-activated transcription factor. A small fraction of progesterone receptor is palmitoylated and ...
  • A set of accessible enhancers enables the initial response of breast cancer cells to physiological progestin concentrations
    Zaurín Quer, Roser; Ferrari, Roberto; Nacht, A. Silvina; Carbonell-Caballero, Jose; Le Dily, François; Font Mateu, Jofre, 1977-; De Llobet, Lara Isabel; Vidal Ocabo, Enrique; Lioutas, Antonio, 1980-; Beato, Miguel; Vincent, Guillermo P. (Oxford University Press, 2021)
    Here, we report that in T47D breast cancer cells 50 pM progestin is sufficient to activate cell cycle entry and the progesterone gene expression program. At this concentration, equivalent to the progesterone blood levels ...
  • Análisis de los dominios funcionales del receptor de progesterona en líneas celulares estables de cáncer de mama
    Quiles Lara, Ignacio (Universitat Pompeu Fabra, 2011-04-12)
    Esta tesis se interesa por distinguir entre los efectos directos de los receptores nucleares y aquellos mediados por las rutas de transducción de señales en la transcripción de genes en respuesta a hormona y proliferación ...
  • Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry
    Reim, Alexander; Ackermann, Roland; Font Mateu, Jofre, 1977-; Kammel, Robert; Beato, Miguel; Nolte, Stefan; Mann, Matthias; Russmann, Christoph; Wierer, Michael, 1982- (Nature Research, 2020)
    Transcription factors (TFs) regulate target genes by specific interactions with DNA sequences. Detecting and understanding these interactions at the molecular level is of fundamental importance in biological and clinical ...
  • ATP, Mg2+, nuclear phase separation, and genome accessibility
    Wright, Roni H.G.; Le Dily, François; Beato, Miguel (Elsevier, 2019)
    Misregulation of the processes controlling eukaryotic gene expression can result in disease. Gene expression is influenced by the surrounding chromatin; hence the nuclear environment is also of vital importance. Recently, ...
  • bwtool: a tool for bigWig files
    Pohl, Andy, 1979-; Beato, Miguel (Oxford University Press, 2014)
    BigWig files are a compressed, indexed, binary format for genome-wide signal data for calculations (e.g. GC percent) or experiments (e.g. ChIP-seq/RNA-seq read depth). bwtool is a tool designed to read bigWig files rapidly ...
  • C/EBPalpha activates pre-existing and de novo macrophage enhancers during induced pre-B cell transdifferentiation and myelopoiesis
    van Oevelen, Chris; Collombet, Samuel; Vicent, Guillermo Pablo; Hoogenkamp, Maarten; Lepoivre, Cyrille; Badeaux, Aimee; Bussmann, Lars; Sardina, Jose Luis; Thieffry, Denis; Beato, Miguel; Shi, Yang; Bonifer, Constanze; Graf, T. (Thomas) (Elsevier, 2015)
    Transcription-factor-induced somatic cell conversions are highly relevant for both basic and clinical research yet their mechanism is not fully understood and it is unclear whether they reflect normal differentiation ...
  • C/EBPα mediates the growth inhibitory effect of progestins on breast cancer cells
    Nacht, A. Silvina; Ferrari, Roberto; Zaurín Quer, Roser; Scabia, Valentina; Carbonell-Caballero, Jose; Le Dily, François; Quilez Oliete, Javier; Leopoldi, Alexandra; Brisken, Cathrin; Beato, Miguel; Vicent, Guillermo Pablo (EMBO Press, 2019)
    Steroid hormones are key gene regulators in breast cancer cells. While estrogens stimulate cell proliferation, progestins activate a single cell cycle followed by proliferation arrest. Here, we use biochemical and genome-wide ...
  • CDK2-dependent activation of PARP-1 is required for hormonal gene regulation in breast cancer cells
    Wright, Roni H.G.; Castellano, Giancarlo; Bonet Martínez, Jaume, 1982-; Le Dily, François; Font Mateu, Jofre, 1977-; Ballaré, Cecilia Julia; Nacht, A. Silvina; Soronellas, Daniel; Oliva Miguel, Baldomero; Beato, Miguel (Cold Spring Harbor Laboratory Press (CSHL Press), 2012)
    Eukaryotic gene regulation implies that transcription factors gain access to genomic information via poorly understood processes involving activation and targeting of kinases, histone-modifying enzymes, and chromatin ...
  • Chromatin and RNA maps reveal regulatory long noncoding RNAs in mouse
    Bogu, Gireesh K., 1984-; Vizán, Pedro; Stanton, Lawrence W.; Beato, Miguel; Di Croce, Luciano; Marti-Renom, Marc A. (American Society for Microbiology, 2015)
    Discovering and classifying long noncoding RNAs (lncRNAs) across all mammalian tissues and cell lines remains a major challenge. Previously, mouse lncRNAs were identified using transcriptome sequencing (RNA-seq) data from ...
  • Chromatin remodeling in Drosophila preblastodermic embryo extract
    Šatović, Eva; Font Mateu, Jofre, 1977-; Carbonell, Albert; Beato, Miguel; Azorín Marín, Fernando (Nature Research, 2018)
    Chromatin is known to undergo extensive remodeling during nuclear reprogramming. However, the factors and mechanisms involved in this remodeling are still poorly understood and current experimental approaches to study it ...
  • Chromatin topology defines estradiol-primed progesterone receptor and PAX2 binding in endometrial cancer cells
    La Greca, Alejandro; Bellora Pereyra, Nicolás; Le Dily, François; Jara, Rodrigo; Nacht, A. Silvina; Quilez Oliete, Javier; Villanueva Cañas, José Luis, 1984-; Vidal Barba, Enric; Merino, Gabriela; Fresno, Cristóbal; Reischle, Inti Tarifa; Vallejo, Griselda; Vicent, Guillermo Pablo; Fernández, Elmer; Beato, Miguel; Saragüeta, Patricia (eLife Sciences Publications, 2022)
    Estrogen (E2) and Progesterone (Pg), via their specific receptors (ERalpha and PR), are major determinants in the development and progression of endometrial carcinomas, However, their precise mechanism of action and the ...
  • Distinct structural transitions of chromatin topological domains correlate with coordinated hormone-induced gene regulation
    Le Dily, François; Baù, Davide; Pohl, Andy, 1979-; Vicent, Guillermo Pablo; Serra, François; Soronellas, Daniel; Castellano, Giancarlo; Wright, Roni H.G.; Ballare, Cecilia Julia; Filion, Guillaume; Marti-Renom, Marc A.; Beato, Miguel (Cold Spring Harbor Laboratory Press (CSHL Press), 2014)
    The human genome is segmented into topologically associating domains (TADs), but the role of this conserved organization during transient changes in gene expression is not known. Here we describe the distribution of ...
  • Dynamics of progesterone receptor interactors in breast cancer cells upon hormone exposure
    Font Mateu, Jofre (Universitat Pompeu Fabra, 2018-04-30)
    El receptor de la progesterona és un regulador clau per la proliferació de les cèl·lules de càncer de mama dependents d’hormona. El mecanisme d’acció del PR ha tingut un paper important en la resolució del mecanisme molecular ...
  • En el umbral de una nueva biología
    Beato, Miguel (Universitat Pompeu Fabra, 1998)
  • Evolutionary analysis reveals the role of a non-catalytic domain of peptidyl arginine deiminase 2 in transcriptional regulation
    Villanueva Cañas, José Luis, 1984-; Fernández Fuentes, Narcís; Saul, Dominik; Kosinsky, Robyn Laura; Teyssier, Catherine; Rogalska, Malgorzata; Pegenaute Pérez, Ferran; Oliva Miguel, Baldomero; Notredame, Cedric; Beato, Miguel; Sharma, Priyanka (Elsevier, 2024)
    Peptidyl arginine deiminases (PADIs) catalyze protein citrullination, a post-translational conversion of arginine to citrulline. The most widely expressed member of this family, PADI2, regulates cellular processes that ...
  • Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
    Pickup, Katherine E.; Pardow, Felicitas; Carbonell-Caballero, Jose; Lioutas, Antonio, 1980-; Villanueva Cañas, José Luis, 1984-; Wright, Roni H.G.; Beato, Miguel (MDPI, 2019)
    The growth of cancer cells as oncospheres in three-dimensional (3D) culture provides a robust cell model for understanding cancer progression, as well as for early drug discovery and validation. We have previously described ...
  • Global signalling network analysis of luminal T47D breast cancer cells in response to progesterone
    Wright, Roni H.G.; Vastolo, Viviana; Quilez Oliete, Javier; Carbonell-Caballero, Jose; Beato, Miguel (Frontiers, 2022)
    Background: Breast cancer cells enter into the cell cycle following progestin exposure by the activation of signalling cascades involving a plethora of enzymes, transcription factors and co-factors that transmit the external ...

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