Visualitza per autoria "Graf, T. (Thomas)"

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  • C/EBPalpha activates pre-existing and de novo macrophage enhancers during induced pre-B cell transdifferentiation and myelopoiesis
    van Oevelen, Chris; Collombet, Samuel; Vicent, Guillermo Pablo; Hoogenkamp, Maarten; Lepoivre, Cyrille; Badeaux, Aimee; Bussmann, Lars; Sardina, Jose Luis; Thieffry, Denis; Beato, Miguel; Shi, Yang; Bonifer, Constanze; Graf, T. (Thomas) (Elsevier, 2015)
    Transcription-factor-induced somatic cell conversions are highly relevant for both basic and clinical research yet their mechanism is not fully understood and it is unclear whether they reflect normal differentiation ...
  • C/EBPα instructs trophectoderm and pluripotency through the II6 pathway
    Plana Carmona, Marcos (2021-04-09)
    Continuous C/EBPa expression converts B cells into macrophages while its transient upregulation before the Yamanaka factors yields highly efficient iPSC reprogramming. C/EBP members regulate Il6 pathway genes and IL-6 ...
  • C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells
    Di Stefano, Bruno (Universitat Pompeu Fabra, 2014-10-31)
    One of the major goals of current stem cell research is understanding the mechanism of somatic cell reprogramming by Oct4, Sox2, Klf4 and Myc (OSKM) into induced pluripotent stem cells (iPSCs). However, the finding that only ...
  • C10-HDAC7
    Barneda Zahonero, Bruna; Román González, Lidia; Collazo, Olga; Rafati, Haleh; Islam, Abul, 1978-; Bussmann, Lars; Di Tullio, Alessandro, 1982-; Andrés, Luisa De; Graf, T. (Thomas); López Bigas, Núria; Mahmoudi, Tokameh; Parra, Maribel (Universitat Pompeu Fabra, 2013-05)
    HDAC7 is a repressor of myeloid genes whose downregulation in pre-B cells is required for transdifferentiation into macrophages.
  • Carm1-arginine methylation of the transcription factor C/EBPα regulates transdifferentiation velocity
    Torcal Garcia, Guillem, 1991-; Kowenz-Leutz, Elisabeth; Tian, Tian V.; Klonizakis, Antonios; Lerner, Jonathan; Andrés Aguayo, Luisa de; Sapozhnikova, Valeriia; Berenguer, Clara; Plana Carmona, Marcos, 1993-; Vila-Casadesús, Maria; Bulteau, Romain; Francesconi, Mirko; Peiró Sales, Sandra; Mertins, Philipp; Zaret, Kenneth S.; Leutz, Achim; Graf, T. (Thomas) (eLife, 2023)
    Here, we describe how the speed of C/EBPα-induced B cell to macrophage transdifferentiation (BMT) can be regulated, using both mouse and human models. The identification of a mutant of C/EBPα (C/EBPαR35A) that greatly ...
  • CEBPA phase separation links transcriptional activity and 3D chromatin hubs
    Christou-Kent, Marie; Cuartero, Sergi; Garcia-Cabau, Carla; Ruehle, Julia; Naderi, Julian; Erber, Julia; Neguembor, Maria Victoria; Plana Carmona, Marcos, 1993-; Alcoverro-Bertran, Marc; Andrés Aguayo, Luisa de; Klonizakis, Antonios; Julià Vilella, Eric; Lynch, Cian J.; Serrano, Manuel; Hnisz, Denes; Salvatella, Xavier; Graf, T. (Thomas); Stik, Grégoire (Elsevier, 2023)
    Cell identity is orchestrated through an interplay between transcription factor (TF) action and genome architecture. The mechanisms used by TFs to shape three-dimensional (3D) genome organization remain incompletely ...
  • Cell-of-origin-specific 3D genome structure acquired during somatic cell reprogramming
    Krijger, Peter Hugo Lodewijk; Di Stefano, Bruno, 1984-; de Wit, Elzo; Limone, Francesco; van Oevelen, Chris; de Laat, Wouter; Graf, T. (Thomas) (Elsevier, 2016)
    Forced expression of reprogramming factors can convert somatic cells into induced pluripotent stem cells (iPSCs). Here we studied genome topology dynamics during reprogramming of different somatic cell types with highly ...
  • Constitutively active SMAD2/3 are broad-scope potentiators of transcription-factor-mediated cellular reprogramming
    Ruetz, Tyson Joel; Pfisterer, Ulrich; Di Stefano, Bruno, 1984-; Ashmore, James; Beniazza, Meryam; Tian, Tian V.; Kaemena, Daniel F.; Tosti, Luca; Tan, Wenfang; Manning, Jonathan R.; Chantzoura, Eleni; Ottosson, Daniella Rylander; Collombet, Samuel; Johnsson, Anna E.; Cohen, Erez; Yusa, Kosuke; Linnarsson, Sten; Graf, T. (Thomas); Parmar, Malin; Kaji, Keisuke (Elsevier, 2017)
    Reprogramming of cellular identity using exogenous expression of transcription factors (TFs) is a powerful and exciting tool for tissue engineering, disease modeling, and regenerative medicine. However, generation of desired ...
  • Covering the stem cell explosion at the 2017 ISSCR Conference in Boston
    Graf, T. (Thomas) (Cell Press, 2017)
    The meeting covered a plethora of rapidly evolving approaches and areas, such as organoid cultures modeling tissues and organs; stem cell-specific metabolites revealing new signaling pathways; single-cell technologies ...
  • CTCF chromatin residence time controls three-dimensional genome organization, gene expression and DNA methylation in pluripotent cells
    Soochit, Widia; Stik, Grégoire; Vidal, Enrique; Graf, T. (Thomas); Galjart, Niels (Nature Research, 2021)
    The 11 zinc finger (ZF) protein CTCF regulates topologically associating domain formation and transcription through selective binding to thousands of genomic sites. Here, we replaced endogenous CTCF in mouse embryonic stem ...
  • CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response
    Stik, Grégoire; Vidal, Enrique; Barrero, Mercedes; Cuartero, Sergi; Vila-Casadesús, Maria; Mendieta Esteban, Julen, 1992-; Tian, Tian V.; Choi, Jinmi; Berenguer, Clara; Abad, Amaya; Borsari, Beatrice, 1992-; Le Dily, François; Cramer, Patrick; Martí Renom, Marc A.; Stadhouders, Ralph; Graf, T. (Thomas) (Nature Research, 2020)
    Three-dimensional organization of the genome is important for transcriptional regulation1-7. In mammals, CTCF and the cohesin complex create submegabase structures with elevated internal chromatin contact frequencies, ...
  • Dynamics of alternative splicing during somatic cell reprogramming reveals functions for RNA-binding proteins CPSF3, hnRNP UL1, and TIA1
    Vivori, Claudia, 1989-; Papasaikas, Panagiotis; Stadhouders, Ralph; Di Stefano, Bruno, 1984-; Ribó Rubio, Anna; Berenguer, Clara; Generoso, Serena Francesca, 1988-; Mallol, Anna; Sardina, Jose Luis; Payer, Bernhard; Graf, T. (Thomas); Valcárcel, J. (Juan) (BioMed Central, 2021)
    Background: Somatic cell reprogramming is the process that allows differentiated cells to revert to a pluripotent state. In contrast to the extensively studied rewiring of epigenetic and transcriptional programs required ...
  • Evidence for additive and synergistic action of mammalian enhancers during cell fate determination
    Choi, Jinmi; Lysakovskaia, Kseniia; Sitk, Gregoire; Demel, Carina; Söding, Johannes; Tian, Tian V.; Graf, T. (Thomas); Cramer, Patrick (eLife Sciences Publications, 2021)
    Enhancer activity drives cell differentiation and cell fate determination, but it remains unclear how enhancers cooperate during these processes. Here we investigate enhancer cooperation during transdifferentiation of human ...
  • HDAC7 is a repressor of myeloid genes whose downregulation is required for transdifferentiation of pre-B cells into macrophages
    Barneda Zahonero, Bruna; Román González, Lidia; Collazo, Olga; Rafati, Haleh; Islam, Abul, 1978-; Bussmann, Lars; Di Tullio, Alessandro, 1982-; Andrés, Luisa De; Graf, T. (Thomas); López Bigas, Núria; Mahmoudi, Tokameh; Parra, Maribel (Public Library of Science (PLoS), 2013)
    B lymphopoiesis is the result of several cell-commitment, lineage-choice, and differentiation processes. Every differentiation step is characterized by the activation of a new, lineage-specific, genetic program and the ...
  • Human induced-T-to-natural killer cells have potent anti-tumour activities
    Jiang, Zhiwu; Hong, Qilan; Graf, T. (Thomas); Li, Peng (BioMed Central, 2022)
    Background: Adoptive cell therapy (ACT) is a particularly promising area of cancer immunotherapy, engineered T and NK cells that express chimeric antigen receptors (CAR) are being explored for treating hematopoietic ...
  • Human Monocytes Engage an Alternative Inflammasome Pathway
    Gaidt, Moritz M.; Ebert, Thomas S.; Chauhan, Dhruv; Schmidt, Tobias; Schmid-Burgk, Jonathan L.; Rapino, Francesca, 1982-; Robertson, Avril A. B.; Cooper, Matthew A.; Graf, T. (Thomas); Hornung, Veit (Elsevier, 2016)
    Interleukin-1β (IL-1β) is a cytokine whose bioactivity is controlled by activation of the inflammasome. However, in response to lipopolysaccharide, human monocytes secrete IL-1β independently of classical inflammasome ...
  • Identification of enhancer-promoter contacts in embryoid bodies by quantitative chromosome conformation capture (4C)
    Tian, Tian V.; Vidal Ocabo, Enrique; Graf, T. (Thomas); Stik, Grégoire (MyJove Corporation, 2020)
    During mammalian development, cell fates are determined through the establishment of regulatory networks that define the specificity, timing, and spatial patterns of gene expression. Embryoid bodies (EBs) derived from ...
  • Induced transdifferentiation of human B-leukemia/lymphoma cell lines and inhibition of leukemogenicity
    Rapino, Francesca (Universitat Pompeu Fabra, 2014-01-14)
    B-cell malignancies encompass a wide variety of distinct diseases including Non Hodgkin lymphoma (NHL) and leukemia. Currently, chemotherapy, radiation and anti-CD20 antibody treatment are the mainstays of B-cell lymphoma ...
  • Knockout of RNA binding protein MSI2 impairs follicle development in the mouse ovary: characterization of MSI1 and MSI2 during folliculogenesis
    Sutherland, Jessie M.; Sobinoff, Alexander P.; Gunter, Kara M.; Fraser, Barbara A.; Pye, Victoria; Bernstein, Ilana R.; Boon, Evan; Siddall, Nicole A.; Andrés, Luisa De; Hime, Gary R.; Holt, Janet E.; Graf, T. (Thomas); McLaughlin, Eileen A. (MDPI, 2015)
    Characterizing the mechanisms underlying follicle development in the ovary is crucial to understanding female fertility and is an area of increasing research interest. The RNA binding protein Musashi is essential for ...
  • Methylation of a lineage-instructive transcription factor acts as a cell fate switch
    Torcal Garcia, Guillem (2022-02-21)
    Lineage-instructive transcription factors orchestrate cell fate decisions by regulating gene expression programs. However, how their function is regulated throughout development is poorly understood. C/EBPα is a transcription ...

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