Barriga de Vicente, Francisco Martín, 1983-Montagni, Elisa, 1984-Mana, MiyekoMéndez Lago, MaríaHernando-Momblona, XavierSevillano, MartaGuillaumet-Adkins, AmyRodríguez Esteban, GustavoBuczacki, Simon J.A.Gut, MartaHeyn, HolgerWinton, Douglas J.Yilmaz, Omer H.Attolini, Camille Stephan-OttoGut, Ivo GlynneBatlle Gómez, Eduard2018-08-212018-08-212017Barriga FM, Montagni E, Mana M, Mendez-Lago M, Hernando-Momblona X, Sevillano M et al. Mex3a marks a slowly dividing subpopulation of Lgr5+ intestinal stem cells. Cell Stem Cell. 2017 June;20(6):801-16. DOI: 10.1016/j.stem.2017.02.0071934-5909http://hdl.handle.net/10230/35344Highly proliferative Lgr5+ stem cells maintain the intestinal epithelium and are thought to be largely homogeneous. Although quiescent intestinal stem cell (ISC) populations have been described, the identity and features of such a population remain controversial. Here we report unanticipated heterogeneity within the Lgr5+ ISC pool. We found that expression of the RNA-binding protein Mex3a labels a slowly cycling subpopulation of Lgr5+ ISCs that contribute to all intestinal lineages with distinct kinetics. Single-cell transcriptome profiling revealed that Lgr5+ cells adopt two discrete states, one of which is defined by a Mex3a expression program and relatively low levels of proliferation genes. During homeostasis, Mex3a+ cells continually shift into the rapidly dividing, self-renewing ISC pool. Chemotherapy and radiation preferentially target rapidly dividing Lgr5+ cells but spare the Mex3a-high/Lgr5+ population, helping to promote regeneration of the intestinal epithelium following toxic insults. Thus, Mex3a defines a reserve-like ISC population within the Lgr5+ compartment.application/pdfeng© Elsevier This is the published version of an article http://dx.doi.org/10.1016/j.stem.2017.02.007 that appeared in the journal Cell Stem Cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: https://www.elsevier.com/about/our-business/policies/open-access-licenses/elsevier-user-licenseCèl·lules -- ProliferacióMucosa intestinalProteïnesCèl·lules mareinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.stem.2017.02.007info:eu-repo/semantics/openAccess