Bigas Salvans, AnnaGalán i Palma, LuisKartha, Gayathri M.Giorgetti, Alessandra2023-05-122023-05-122022Bigas A, Galán Palma L, Kartha GM, Giorgetti A. Using pluripotent stem cells to understand normal and leukemic hematopoietic development. Stem Cells Transl Med. 2022 Nov 18;11(11):1123-34. DOI: 10.1093/stcltm/szac0712157-6564http://hdl.handle.net/10230/56788Several decades have passed since the generation of the first embryonic stem cell (ESC) lines both in mice and in humans. Since then, stem cell biologists have tried to understand their potential biological and clinical uses for their implementation in regenerative medicine. The hematopoietic field was a pioneer in establishing the potential use for the development of blood cell products and clinical applications; however, early expectations have been truncated by the difficulty in generating bonafide hematopoietic stem cells (HSCs). Despite some progress in understanding the origin of HSCs during embryonic development, the reproduction of this process in vitro is still not possible, but the knowledge acquired in the embryo is slowly being implemented for mouse and human pluripotent stem cells (PSCs). In contrast, ESC-derived hematopoietic cells may recapitulate some leukemic transformation processes when exposed to oncogenic drivers. This would be especially useful to model prenatal leukemia development or other leukemia-predisposing syndromes, which are difficult to study. In this review, we will review the state of the art of the use of PSCs as a model for hematopoietic and leukemia development.application/pdfeng© The Author(s) 2022. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.cominfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/stcltm/szac071Adult hematopoietic stem cellsEmbryoEmbryonic stem cells (ESCs)Hematologic malignanciesPluripotent stem cellsinfo:eu-repo/semantics/openAccess