Ricci, Maria Aurelia, 1985-Cosma, Maria Pia, 1970-Lakadamyali, Melike2019-04-252019-04-252017Ricci MA, Cosma MP, Lakadamyali M. Super resolution imaging of chromatin in pluripotency, differentiation, and reprogramming. Curr Opin Genet Dev. 2017;46:186-93. DOI 10.1016/j.gde.2017.07.0100959-437Xhttp://hdl.handle.net/10230/37129Chromatin fiber organization is essential for gene function in all cell types. Moreover it helps to determine cell fate in embryonic/adult stem cells and in somatic cells undergoing reprogramming to pluripotency. Until now the diffraction limit of light has limited the inspection of the chromatin fiber organization to a level sufficient to understand how it impacts gene function. The development of advanced microscopy methods, such as single molecule localization microscopy, has largely opened a new field of research providing us with the tools to visualize and quantitatively analyze chromatin fiber organization and thus gene activity at nanoscale resolution in single cells.application/pdfeng© Elsevier http://dx.doi.org/10.1016/j.gde.2017.07.010info:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.gde.2017.07.010info:eu-repo/semantics/openAccess