Bevilacqua, CarloGomez, Juan ManuelFiuza, Ulla-MajChan, Chii JouWang, LingHambura, SebastianEguren, ManuelEllenberg, JanDiz-Muñoz, AlbaLeptin, MariaPrevedel, Robert2023-06-122023-06-122023Bevilacqua C, Gomez JM, Fiuza U, Chan CJ, Wang L, Hambura S, et al. High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development. Nat Methods. 2023 May;20:755-60. DOI: 10.1038/s41592-023-01822-11548-7091http://hdl.handle.net/10230/57145Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.application/pdfengThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.MicroscòpiaImatgeria tridimensionalBiologia -- Aparells i instrumentsEmbrionsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41592-023-01822-1info:eu-repo/semantics/openAccess